基于反应的放射测量传感器,用于使用自发拉曼散射在活细胞中同时进行多生物分析成像
Sujit K Das1, Heqi Xi2, Itsuki Yamamoto2
1Department of Chemical Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, 400005, India.
Angewandte Chemie (International ed. in English)
|December 29, 2025
概括
研究人员开发了用于敏感生物分析成像的新型拉曼响应比度传感器 (ABATaRs). 这些探头克服了灵敏度的局限性,使用自发拉曼显微镜在活细胞中实现更快,多分析成像.
科学领域:
- 化学生物学 化学生物学
- 频谱学是一种光谱学.
- 生物成像是一种生物成像.
背景情况:
- 拉曼散射提供了多分析器成像潜力,由于狭窄的峰值宽度.
- 基/基标签对于拉曼探测器至关重要,但由于拉曼散射横截面较低,限制了灵敏度.
- 现有的拉曼响应式比度传感器在可访问的自发拉曼平台上难以获得灵敏度.
研究的目的:
- 为自发的拉曼成像开发高度敏感的拉曼响应式比度传感器.
- 为了克服与基因/酸拉曼探针相关的低灵敏度挑战.
- 为了证明这些传感器在活细胞中成像各种生物分析物的多功能性.
主要方法:
- 利用"推拉"电子效应来设计基于活动的基因标记拉曼传感器 (ABATaRs).
- 与基准相比,量化计算和实验拉曼散射活动和相对横截面.
- 为pH,过氧化和铜 (Cu) 离子开发了细胞透的比度ABATaR.
主要成果:
- ABATaRs的拉曼散射活动 (12-38倍) 和实验拉曼强度 (5-22倍) 显著高于基准EdU.
- 实现了高相对拉曼散射横截面用于基因拉伸 (高达466对比DMSO C-H拉伸).
- 通过自发拉曼显微镜,在低传感器度 (1-5μM) 的活细胞中成功成像了生物分析物 (pH,H2O2,Cu离子) 的生理和病理生理水平.
- 证明了活细胞中离子和过氧化的同时多分析拉曼成像,提高了成像速度.
结论:
- 在可访问的自发拉曼平台上,ABATaRs代表了基于拉曼的敏感生物分析成像的突破.
- 开发的传感器为各种分析物提供了高灵敏度,比度检测和多功能性.
- ABATaR显著提升了活细胞多分析仪成像能力,使得检测更快,更灵敏.
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