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炎症性肠病与肠外染色体循环DNA增加有关:IBD类型和活动的新兴生物标志物
Valentina Petito1, Daniela Gerovska2, Antonia Piazzesi3
1CeMAD Translational Research Laboratories, Digestive Disease Center (CeMAD), Department of Medical and Surgical Sciences, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Rome, Italy.
Journal of Crohn's & colitis
|December 29, 2025
概括
外染色体圆形DNA (eccDNA) 在炎症性肠病 (IBD) 患者中升高. 特定的eccDNA模式可以作为IBD诊断和疾病活动的新生物标志物.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 炎症性肠病 (IBD) 是一种慢性胃肠道自身免疫性疾病,临床过程可变.
- 目前IBD的诊断方法包括侵入性手术和临床评估.
- 在IBD中治疗反应率有限,这凸显了需要可靠的疾病标志物和预测因素的需要.
研究的目的:
- 研究染色体外圆形DNA (eccDNA) 在炎症性肠病 (IBD) 中的存在和潜在作用.
- 探索eccDNA作为IBD诊断,疾病活性和治疗反应的潜在生物标志物.
- 确定与不同IBD亚型和疾病状态相关的特定eccDNA模式.
主要方法:
- 从IBD患者和健康对照中收集肠道活检.
- 循环DNA的丰富,测序和识别,使用循环寻找器和床具.
- 根据IBD类型 (性结肠炎,克罗恩病) 和疾病活性 (活性与缓解) 的患者分层.
主要成果:
- 与健康对照人群相比,IBD患者的肠道组织中检测到显著更高的eccDNA水平.
- 与缓解期患者相比,在活跃的性结肠炎患者中观察到eccDNA产生的增加.
- IBD患者表现出包含特定基因片段的一致eccDNA模式,与对照组的随机模式形成鲜明对比.
结论:
- 在肠道活检中确定了IBD特异性的eccDNA生产模式.
- 在eccDNA中的特定基因热点与IBD中的活性疾病状态相关.
- 含有基因碎片的eccDNAs,特别是NRG1和ZPMF2等基因碎片,显示出作为IBD的新生物标志物具有前途.
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