一个冷链自由循环介导的同热放大测试,用于检测人类疟疾在 point-of-care 设置
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Updated: Jul 22, 2026

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A Multi-detection Assay for Malaria Transmitting Mosquitoes
Published on: February 28, 2015
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Nurul Nabila Rosli1, Yee Ling Lau1, Meng Yee Lai1
1Department of Parasitology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Diagnostic microbiology and infectious disease
|December 29, 2025
概括
一个新的疟疾诊断测试使用基于糖的保存来保持室温稳定性. 这种具有成本效益,无冷链的方法可以在偏远地区可靠地检测Plasmodium knowlesi.
科学领域:
- 分子诊断学 分子诊断学
- 寄生虫学的寄生虫学
- 生物化学 生物化学
背景情况:
- 在资源有限的环境中诊断疟疾需要强大的,无冷链的分子测试.
- 传统的循环介导同热放大 (LAMP) 测试由于冷链要求而面临基础设施挑战.
研究的目的:
- 开发一种独立于制冷的LAMP试验,用于检测Plasmodium knowlesi.
- 为了评估LAMP试剂的室温稳定性,以糖为基础的酶保存.
主要方法:
- 研究了Bst DNA聚合酶的基于糖的酶保存 (糖糖,葡萄糖,银糖).
- 评估了在室温下保存糖的LAMP试剂的稳定性和性能.
- 确定优化试验的分析灵敏度和特异性.
主要成果:
- 糖在室温下有效地保留了Bst DNA聚合酶活性,表现优于葡萄糖和银河糖.
- 保存糖的LAMP试验在12天内保持了一致的性能.
- 该试验显示了高分析灵敏度 (10拷贝/μL) 和100%的特异性.
结论:
- 一种具有成本效益的,基于糖的化学保存方法为LAMP测定提供了一种可行的冷化替代方案.
- 12天的室温稳定性适用于远程诊所的现场部署.
- 这种测试是理想的疟疾监测和诊断在低资源的设置.
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Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
Assessing Body Temperature - Rectal
Rectal temperature measurement is considered the most precise method for assessing core body temperature and typically registers higher than oral temperature. For adults, the rectal thermometer should be inserted 1 to 1.5 inches into the rectum to obtain the most accurate reading.
Follow these steps for rectal temperature assessment:
Step 1: Perform hand hygiene and don clean gloves to prevent cross-infection.
Step 2: Position the patient in a side-lying position to better visualize the rectal...
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Step 1: Perform hand hygiene and don clean gloves to prevent cross-infection.
Step 2: Position the patient in a side-lying position to better visualize the rectal...
Assessing Body Temperature - Axilla
Procedural Guide for Assessing Axillary Body Temperature using a Digital Thermometer:
Step 1: Perform hand hygiene and put on clean gloves to maintain infection control and prevent cross-contamination.
Step 2: Prepare the patient by explaining the procedure to ensure understanding and cooperation. Ensure privacy, expose the axilla, and inform the patient that minimal movement is crucial for an accurate reading.
Step 3: Adjust the patient’s clothing to expose only the axilla. It minimizes...
Step 1: Perform hand hygiene and put on clean gloves to maintain infection control and prevent cross-contamination.
Step 2: Prepare the patient by explaining the procedure to ensure understanding and cooperation. Ensure privacy, expose the axilla, and inform the patient that minimal movement is crucial for an accurate reading.
Step 3: Adjust the patient’s clothing to expose only the axilla. It minimizes...

