长非编码RNAMALAT1编码了一种微,通过抑制先天免疫反应来促进流感A病毒的复制
Kul Raj Rai1, Faxin Wen1, Mohamed Maarouf1
1Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
流感A型病毒感染上调长非编码RNAMALAT1,抑制先天免疫力. 一个MALAT1编码的微增强了病毒复制,揭示了病毒免疫逃避策略.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 长非编码RNAs (lncRNAs) 是生物过程的关键调节者.
- 病毒感染调节宿主lncRNA的表达,影响诸如天生的免疫力之类的细胞通路.
- 在A型流感病毒 (IAV) 感染中,MALAT1 (一个关键的lncRNA) 的作用在很大程度上是未知的.
研究的目的:
- 在IAV感染期间调查MALAT1调节.
- 确定MALAT1在IAV病原发生中的作用.
- 确定涉及MALAT1.1的病毒免疫逃避机制.
主要方法:
- 在IAV感染的细胞和动物中对MALAT1表达的定量分析.
- 通过NF-κB/IL-6/STAT3途径对MALAT1调节的研究.
- 功能性研究涉及MALAT1敲击和过度表达.
- 使用核糖体分析和质谱学识别MALAT1编码的微.
主要成果:
- IAV感染强烈上调MALAT1表达,独立于干扰素信号传递.
- 马拉特1的表达是由NF-κB/IL-6/STAT3通路调节的.
- 破坏MALAT1抑制了IAV复制;碎片增强了它.
- 一个由MALAT1编码的52氨基酸微 (miPEP-52) 增强了IAV复制.
- 马拉特1和miPEP-52抑制了对IAV的先天免疫反应.
结论:
- 马拉特1在IAV感染和发病过程中起着重要作用.
- 编码在MALAT1中的miPEP-52增强了IAV的复制.
- 马拉特1通过抑制天生的免疫力,促进病毒免疫逃避.
更多相关视频
07:24Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
Published on: December 13, 2018
11:20Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
Published on: June 3, 2012
相关概念视频
Leaky Scanning
lncRNA - Long Non-coding RNAs
lncRNA - Long Non-coding RNAs
siRNA - Small Interfering RNAs
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs
