腺三酸盐以度依赖的方式促进α-Synuclein的粉样蛋白形成
Keiichi Yamaguchi1, Maya Sawada2, Kichitaro Nakajima1
1Graduate School of Engineering, The University of Osaka, Suita, Osaka 565-0871, Japan.
Langmuir : the ACS journal of surfaces and colloids
|December 29, 2025
概括
腺三酸盐 (ATP) 和类似的分子通过电荷相互作用和盐分效应加速粉样蛋白的形成. 这些发现揭示了ATP的存在.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 生物物理学的生物物理.
背景情况:
- 氨酸三酸盐 (ATP) 对于生物过程至关重要,可以充当水基.
- 多酸盐,包括三酸盐,已被证明可以在超声波下加速粉样蛋白的形成.
- 腺核酸在α-synuclein (αSyn) amyloidogenesis中的作用需要进一步阐明.
研究的目的:
- 在超声波下研究由腺核酸 (AMP,ADP,ATP) 影响的α-synuclein粉样蛋白形成的机制.
- 探索腺核酸对氨基基基因的度依赖性影响.
- 为了检查pH在ATP介导蛋白质聚合中的作用.
主要方法:
- 超声波诱导的粉样蛋白形成试验.
- 不同度的腺核酸 (AMP,ADP,ATP) 和多酸盐.
- 使用α-synuclein,β2-microglobulin和胰岛素进行pH依赖的聚合研究.
- 对蛋白质构造变化的分析.
主要成果:
- 在低度和高度下,氨酸核酸通过电荷-电荷相互作用和盐分效应分别加速了αSyn的氨基化形成.
- 与多酸盐观察到类似的度依赖的效应.
- 在酸性pH下,ATP促进了对多种蛋白质的粉样纤维和无形聚合物形成,可能诱导类似于化球体的状态.
结论:
- 通过内部和分子间相互作用,ATP充当盐,稳定紧的蛋白质构造物,包括与原生蛋白相似的结构和粉样纤维.
- 通过破坏蛋白质的超和状态,ATP可能会启动粉样蛋白的形成.
- 腺氨酸核酸在调节蛋白质氨基氨基生成方面表现出双重度依赖的作用.
相关概念视频
03:03Amyloid Fibrils
11.5K
Amyloid fibrils are aggregates of misfolded proteins. Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils.
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...
11.5K
01:22Alzheimer's Disease: Treatment
744
Alzheimer's Disease (AD), a neurodegenerative disorder, is pathologically identified by amyloid plaques and neurofibrillary tangles composed of tau protein. AD pharmacotherapy aims to manage cognitive symptoms, delay disease progression, and treat behavioral symptoms. The treatment is primarily symptomatic and palliative, with no definitive disease-modifying therapy available. Cholinesterase inhibitors, including donepezil (Aricept), rivastigmine (Exelon), and galantamine (Razadyne), are...
744
01:29Drugs Affecting Neurotransmitter Synthesis
2.1K
Drugs affecting neurotransmitter synthesis can impact the adrenergic neuron and the synthesis of neurotransmitters. For example, α-methyltyrosine and carbidopa target specific enzymes involved in catecholamine synthesis. α-methyltyrosine inhibits the enzyme tyrosine hydroxylase, which converts tyrosine into dopamine. By blocking this enzyme, α-methyltyrosine reduces dopamine production and other catecholamines. Carbidopa, on the other hand, inhibits the enzyme dopa decarboxylase,...
2.1K