百合素和SKQ1通过AMPK/Nrf2通路在17°C储存期间改善野猪精子质量
Miaolian Peng1,2, Pengyao Wang1, Yongchang Lu1
1Guangdong Laboratory for Lingnan Modern Agriculture, State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Antioxidants (Basel, Switzerland)
|December 30, 2025
概括
这项研究发现,结合莱科和SKQ1抗氧化剂可以显著改善冷藏期间的猪精质量. 这种组合可以提高精子的活力,完整性和能量代谢,同时减少氧化压力.
科学领域:
- 生殖生物学 生殖生物学
- 氧化压力研究研究 氧化压力研究
- 抗氧化剂治疗是一种抗氧化疗法.
背景情况:
- 在17°C的猪精存储会产生活性氧物种 (ROS),导致线粒体功能障碍和精子损伤.
- 研究了天然和向抗氧化剂,以抵消精子保存期间ROS诱导的损伤.
研究的目的:
- 评估单独或组合使用的柳科和SKQ1在17°C储存期间保护野猪精子质量的有效性.
- 为了研究莱科和SKQ1对精子参数和潜在分子机制的协同作用.
主要方法:
- 猪精液被用不同度的柳科和SKQ1处理,并选择了最佳剂量用于联合应用.
- 评估了精子的活力,运动性,体完整性,膜完整性,ROS,甲基 (MDA),超氧化解酶 (SOD),氨酸过氧化酶 (GSP) 和腺三酸盐 (ATP) 的水平.
- 代谢学和西式涂抹被用于分析差异代谢物和蛋白质表达 (p-AMPK, Nrf2).
主要成果:
- 精子运动性得到了显著的改善,仅用20μM的柳科和50nM的SKQ1.
- 20μM和25nMSKQ1的组合显示出协同作用,增强精子活力,完整性,SOD,GSP和ATP水平.
- 联合治疗显著降低了ROS和MDA水平,改变了与能量代谢和信号转导相关的52种代谢物,并增加了p-AMPK和Nrf2表达.
结论:
- 柳科和SKQ1的组合在17°C储存期间协同改善野猪精子质量.
- 这种改善是通过增强能量代谢和减少氧化应激来实现的,可能是通过激活AMPK/Nrf2通路来实现的.
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