从加热烟草制品和参考香烟的排放对人类肺上皮细胞BEAS-2B细胞中的基因表达和线粒体功能的影响
Suin Park1, Miil Kim1, Wei Jin1
1BK21 FOUR Team and Integrated Research Institute for Drug Development, College of Pharmacy, Dongguk University, Goyang 10326, Gyeonggi-do, Republic of Korea.
Antioxidants (Basel, Switzerland)
|December 30, 2025
概括
加热烟草产品 (HTP) 的基因表达变化比传统香烟弱,但两者都会损害线粒体功能. 两者都增加了线粒体的活性氧物种 (ROS),这表明一种常见的毒性途径.
科学领域:
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 加热烟草制品 (HTP) 被宣传为传统香烟的不太有害的替代品.
- 需要进一步调查HTP的毒理效应.
研究的目的:
- 为了比较HTP排放和传统香烟烟雾对人类肺细胞基因表达和线粒体功能的影响.
- 阐明HTP与传统香烟之间潜在的毒性机制.
主要方法:
- 人类肺上皮细胞 BEAS-2B 被暴露于来自HTP和参考香烟的整个香烟烟雾缩物 (WCSC).
- 通过RNA测序进行了转录组分析,以分析基因表达变化.
- 线粒体功能测试评估了反应性氧物种 (ROS) 生产,ATP水平,膜潜力和形态.
主要成果:
- 与传统香烟 WCSC 相比,HTP WCSC 诱导的基因表达变化不那么明显.
- 无论是HTP还是传统香烟的暴露都降低了氧化酸化 (OXPHOS) 基因表达的调节.
- 在HTP和传统香烟暴露后,线粒体ROS水平增加,而其他线粒体参数基本保持不变.
结论:
- 虽然HTP排放导致的转录基因变化比传统香烟少,但两者都会影响线粒体功能.
- 抑制的OXPHOS基因表达和高的线粒体ROS表明共享的毒性机制.
- 线粒体功能障碍可能是各种烟草制品毒性的常见途径.
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