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马射线和紫外线辐射对人类血液的协同性基因毒性影响

Angeliki Gkikoudi^1,2, Athanasia Adamopoulou¹, Despoina Diamadaki¹

¹DNA Damage Laboratory, Physics Department, School of Applied Mathematical and Physical Sciences, National Technical University of Athens (NTUA), Zografou Campus, 15780 Athens, Greece.

Antioxidants (Basel, Switzerland)

|December 30, 2025

概括

来自马射线和紫外线B (UVB) 的联合辐射暴露显著损害了人类细胞中的基因组稳定性. 这项研究开发了一个模型来评估混合辐射暴露导致的癌症风险增加.

科学领域:

辐射生物学辐射生物学
基因毒理学基因毒理学
细胞和分子生物学是细胞和分子生物学.

背景情况:

对电离 (例如,玛射线) 和非电离 (例如,UVB) 辐射的环境和临床暴露对基因组稳定性构成风险.
联合辐射暴露可能对细胞健康产生协同作用或附加有害影响.

研究的目的:

研究玛辐射和UVB暴露对人类外周血液单核细胞 (PBMCs) 的综合影响.
开发一种可预测的生物物理模型,用于估计混合暴露的辐射等价剂量.
评估组合暴露对基因组不稳定性和细胞增殖的影响.

主要方法:

全血和分离的PBMCs的ex vivo暴露在玛射线和/或UVB.
分析γH2AX焦点形成和染色体异常作为DNA损伤的生物标志物.
细胞增殖测试用于评估暴露后的淋巴细胞活力.
基于二心收益率的预测生物物理模型的开发.

主要成果:

与单个暴露相比,马辐射和紫外线辐射的联合暴露诱导了显著更高的γH2AX焦点和染色体异常水平.
孤立的PBMC比全血样本更明显的DNA损伤.
一些淋巴细胞在UVB或联合暴露后表现出受损的增殖.
开发的模型估计,由于共同暴露,终身癌症风险增加了约9%.

结论:

混合辐射暴露,特别是马射线和UVB,协同增加基因组不稳定性.
计算组合暴露对于准确的基因毒性风险评估和辐射保护策略至关重要.
预测模型为量化与混合辐射场景相关的风险提供了一个框架.

关键词:

紫外线 (UVB) 辐射是什么?抗氧化剂防御抗氧化剂防御染色体异常是一种染色体异常.玛辐射是一种玛辐射.有毒基因的协同作用.氧化应激是一种氧化应激.辐射生物对称学氧化还原失衡氧化还原失衡维修指数维修指数

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