相关实验视频

Updated: Jan 7, 2026

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

增长因子为主的WJ-MSC秘密基因增强了纤维细胞在体外扩张

Katia Jarquín-Yáñez¹, Diana Michel Aguilar-Sandoval¹, Gabriela Piñón-Zárate¹

¹Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico (UNAM), Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, Mexico.

|December 30, 2025

概括

经过优化后的中酶体干细胞分泌物促进了伤口愈合. 一个度为1.25毫克/毫升的刺激沃顿.

科学领域:

再生医学是一种再生医学.
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

介质干细胞 (MSC) 秘密体在再生医学中被探索用于伤口愈合.
沃顿的果MSC (WJ-MSC) 提供了一个可行的来源,用于秘密的生产.
使用FGF和EGF的刺激优化了WJ-MSC分泌基因的增殖和抗衰老特性.

研究的目的:

为了评估WJ-MSC分泌物的疗效,刺激与生长因子,在人体纤维细胞的增殖和伤口关闭 in vitro.
为了确定刺激WJ-MSC分泌的最佳度,用于治疗治疗伤口愈合的治疗应用.

主要方法:

收集并表征了WJ-MSC分泌基因,包括受刺激的 (W FGF/EGF) 和未受刺激的 (WO FGF/EGF).
通过流细胞计和LEGENDplex评估WJ-MSC免疫类型和量化生长因子含量.
培养的人类纤维细胞并用不同度 (1.25 mg/mL和 6.25 mg/mL) 的机密体进行处理,以评估增殖,新陈代谢和伤口关闭.

主要成果:

刺激后WJ-MSC免疫类型保持稳定;生长因子的产生显著增加.
增强纤维细胞增殖和体外伤口关闭的最佳度是1.25 mg/mL刺激的WJ-MSC分泌体.
在同等度下,刺激的分泌体表现出比未刺激的分泌体更高的疗效.

结论:

基和基刺激有效地增强了WJ-MSC分泌体中的生长因子分泌.
刺激WJ-MSC分泌物的1.25 mg/mL度是促进纤维细胞增殖和伤口关闭的最佳水平.
优化WJ-MSC分泌体为再生医学和伤口愈合应用提供了一个有前途的非细胞治疗策略.

关键词:

皮肤表皮生长因子 (EGF)纤维细胞生长因子 (FGF)一些秘密一些秘密.沃顿的果细胞 (WJ-MSC) 是一种

更多相关视频

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Published on: January 7, 2015

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Published on: February 24, 2021

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...