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在非小细胞肺癌患者的液体活检中,miRNA-155-3p和miRNA-3196作为潜在的生物标志物

Daniela Alexandre^1,2,3, Joana Polido¹, Salete Valente⁴

¹RISE-Health, Department of Chemistry, Faculty of Sciences, University of Beira Interior, Rua Marquês d'Ávila e Bolama, 6201-001 Covilhã, Portugal.

|December 30, 2025

概括

在外周血液单核细胞 (PBMC) 中循环的microRNAs (miRs) miR-155-3p和miR-3196显示出对非小细胞肺癌 (NSCLC) 查和监测的潜力. miR-155-3p水平的正常化与改善的生存率相关,这表明预后价值.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
生物标志物发现发现

背景情况:

晚期诊断非小细胞肺癌 (NSCLC) 阻碍了有效的治疗.
非侵入性生物标志物对于早期检测,患者分层和NSCLC治疗监测至关重要.

研究的目的:

评估外周血液单核细胞 (PBMC) 中循环的microRNAs (miRs) miR-155和miR-3196作为NSCLC潜在的非侵入性生物标志物.
评估它们在诊断,患者分层,治疗监测和预后方面的有用性.

主要方法:

从136名NSCLC患者和64名健康捐赠者的PBMC中分离RNA.
定量逆转录PCR (RT-qPCR) 用于测量miR-155-3p,miR-3196和miR-155-5p的表达.
接收器操作特征 (ROC) 分析用于诊断性能和Kaplan-Meier/Cox方法用于生存分析.

主要成果:

与对照组相比,在NSCLC患者中,miR-155-3p是上调的,miR-3196是下调的,AUC分别为0.881和0.784.
在腺癌中,miR-155-3p水平与晚期,转移和吸烟史相关.
在全身治疗期间,循环中的miR水平倾向于正常化,miR-155-3p水平的正常化与改善的整体存活率有关.

结论:

在PBMC中循环的miR-155-3p和miR-3196显示为NSCLC查和监测的非侵入性候选物.
这些miRs可能不足以作为当前值的独立NSCLC诊断;将它们与其他生物标志物结合并调整值可以提高特异性.
为了临床实施,需要在大型前性研究中进一步验证.

关键词:

生物标志物生物标志物临床病理学特征临床病理学特征这种微型RNA-155-3ppp.微型RNA-3196 是什么意思非小细胞肺癌的肺癌.周围血液中的单核细胞.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...