作为COPD的非侵入性生物标志物的液外体微RNA:一个横截面研究
Ourania S Kotsiou1, Irene Tsilioni2,3, Aikaterini Tsingene4
1Laboratory of Human Pathophysiology, Department of Nursing, University of Thessaly, 41500 Larissa, Greece.
唾液外体微RNAs (miRNAs) 在慢性阻塞性肺病 (COPD) 中显示出不同的模式. 像miR-155,miR-126和miR-146a这样的特定miRNA可以帮助诊断COPD并非侵入性地评估其严重程度.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 肺部病理学 肺部病理学
背景情况:
- 慢性阻塞性肺病 (COPD) 的特点是气道炎症和空气流量限制.
- 外体微RNA (exo-miRNA) 正在成为呼吸道分子变化的稳定生物标志物.
- 与血清或支气管支气管洗液相比,COPD的唾液衍生的外微RNA概况在很大程度上仍未被探索.
研究的目的:
- 调查液衍生的外体微RNAs作为诊断和表型化COPD的非侵入性生物标志物的潜力.
- 为了确定与COPD相关的特定的外微RNA特征及其与疾病特征的相关性.
主要方法:
- 诱导的唾液从稳定的COPD患者和健康对照组中收集.
- 异构体被分离并使用传输电子显微镜和西方涂抹进行了表征.
- 使用定量实时PCR (RT-qPCR) 来测量9个候选miRNAs的水平.
- 统计分析包括曼-惠特尼U测试,皮尔森相关性和接收器操作特征 (ROC) 曲线分析.
主要成果:
- 唾液外基因组表现出特有的形态和蛋白质标记物.
- 在COPD患者中观察到几种exo-miRNAs的显著失调,包括miR-21和miR-223的上调和miR-155,miR-126和miR-146a的下调.
- miR-21与症状负担相关 (CAT得分).
- miR-155显示了COPD检测的最高诊断性能 (AUC = 0.730).
- 一个由miR-155,miR-126和miR-146组成的组合面板提高了诊断准确度 (AUC = 0.841).
- miR-126有效地区分了轻度和中度至重度的COPD (AUC = 0.728).
结论:
- 唾液外体miRNAs呈现出一种独特的COPD特异性的分子特征.
- 这种特征反映了潜在的呼吸道炎症,受损的修复机制和COPD的免疫失调.
- 特定的外微RNA (miR-155,miR-126,miR-146a) 的复合面板为非侵入性COPD诊断和临床分期提供了有前途的潜力.
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