细菌的隔离和益生菌功能及其对大肠炎的抑制作用
Ningning Guan1,2, Chang Li1,3, Wei Liu1,3
1Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Biology
|December 30, 2025
概括
来自山羊养殖场的新分离的Bacillus subtilis菌株对恶劣条件表现出了显著的耐受性,并具有抗炎性质. 这种益生菌显示出改善牲畜和家禽肠道健康的潜力.
科学领域:
- 微生物学 微生物学
- 动物科学动物科学
- 胃肠病学 胃肠病学
背景情况:
- 细菌细菌是一种公认的益生菌料添加剂,用于牲畜和家禽.
- 为了提高环境耐受性和有效性,研究新型菌株对于农业应用至关重要.
研究的目的:
- 评估从一家山羊养殖场中分离出来的Bacillus subtilis菌株的环境耐受性和益生菌潜力.
- 在大肠炎的小鼠模型中评估这种菌株的抗炎作用.
主要方法:
- 环境耐受性测试 (温度,pH,胆盐).
- 全基因组测序和功能注释.
- 牛硫酸 (DSS) 诱导的大肠炎的小鼠模型,以评估抗炎作用.
主要成果:
- 隔离的Bacillus subtilis菌株对极端温度,pH值和胆汁盐具有很高的耐受性.
- 基因组测序确定了与代谢和生物合成途径相关的基因.
- 该菌株的口服治疗逆转了DSS诱导的损伤,增加了紧结蛋白 (ZO-1,Occludin) 和降低了促炎细胞因子.
结论:
- 从山羊养殖场分离出来的Bacillus subtilis菌株表现出强大的环境耐受性和显著的抗炎作用.
- 这种菌株作为增强肠道健康和减轻畜牧业炎症的益生菌具有相当的前景.
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