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一个基于3DColMA的原性微环境揭示了来自肌病外科开拓者的肌干细胞/祖细胞 (TSPCs) 的行为

Giacomo Cortella¹, Erwin Pavel Lamparelli¹, Joseph Lovecchio²

¹Department of Medicine, Surgery and Dentistry, University of Salerno, Via S. Allende, 84081 Baronissi, SA, Italy.

Bioengineering (Basel, Switzerland)

|December 30, 2025

概括

这项研究介绍了一种3D生物打印的脚手架,使用甲化I型原蛋白和GDF-5纳米颗粒来建模肌损伤. 该平台有效地复制了人肌病理性干细胞行为和炎症在体外.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
组织工程是组织工程.

背景情况:

肌损伤由于愈合不良和复杂的病理学,造成了重大的临床挑战.
病变性肌干细胞/原生细胞 (hTSPCs) 显示出细胞外基质 (ECM) 沉积和促炎信号的受损.

研究的目的:

开发一个具有持续增长因子释放的3D生物打印支架,用于建模原微环境.
在体外研究病理性hTSPCs的ECM重塑和炎症反应.

主要方法:

通过微流体辅助纳米沉制造增长差异化因子-5 (GDF-5) 装载的多 (乳糖-协同甘油酸) (PLGA) 纳米颗粒.
开发一个3D生物打印的甲基化I型原体 (ColMA) 脚手架,结合GDF-5纳米粒子.
病理性hTSPCs的动态培养在21天内在连续 perfusion (1毫升/分钟) 下的脚手架内.

主要成果:

输液培养维持了细胞活力 (>70%) 并支持ECM沉积.
病理性hTSPCs显示ECM重塑受损,III型原蛋白增加和纤维状的表型.
观察到莫杜林分泌量升高和促炎性细胞因子 (IL-6,IL-8) 概况,与慢性炎症一致.

结论:

模块化的3D生物打印平台有效地模拟了tenogenic微环境和病理细胞行为在体外.
这种模型作为一种有价值的工具,用于慢性肌疾病的机制研究.
该平台支持推进用于肌修复的个性化再生策略.

关键词:

在3D生物打印中使用3D生物打印装有 GDF-5 的 PLGA 纳米粒子.perfusion 生物反应器是一种生物反应器.肌病的建模肌病模型肌干细胞/原生细胞 (hTSPCs) 是一种

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