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使用基于微阵列的系统在阳性血液培养中检测来自格兰阴性细菌的ARG:朝着分子抗生素敏感性检测

Cataldo Maria Mannavola¹, Giordana Cafaro², Barbara Fiori²

¹Department of Basic Biotechnological Sciences, Intensive and Perioperative Clinics, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.

Antibiotics (Basel, Switzerland)

|December 30, 2025

概括

HybriSpot12 PCR AUTO (HS12a) 系统和MDR直流芯片 (MDR-FC) 测试显示,从阳性血液培养物中检测出格兰阴性细菌中的抗菌素耐药基因 (ARG) 的高精度. 虽然对宏酸和β-乳酸盐耐药性有效,但对于检测氨基糖化物耐药性需要进一步改进.

科学领域:

临床微生物学和传染病.
分子诊断和遗传分析
抗微生物药物耐药性的监测监测

背景情况:

抗菌素耐药性 (AMR) 是一个关键的全球健康威胁,需要快速的诊断解决方案.
分子测定,包括多重PCR和DNA微阵列,正在推进细菌识别和抗微生物耐药性基因 (ARG) 检测.

研究的目的:

评估HybriSpot12 PCR AUTO (HS12a) 系统和MDR直流芯片 (MDR-FC) 试验的性能,用于直接从阳性血液培养 (PBC) 样本中检测ARG.
为了评估这种自动化微阵列测定与全基因组测序 (WGS) 标志性隔离物的准确性.

主要方法:

分析了111种格拉姆阴性细菌分离物 (Enterobacterales,Acinetobacter baumannii,Pseudomonas spp.) 的情况. 具有WGS的特点.
从这些分离物中生成了阳性血液培养 (PBC) 样本.
利用HS12a/MDR-FC测定,一种自动反杂交微阵列,用于在PBC样本中检测ARG.

主要成果:

在所有测试物种中,对宏类耐药基因观察到完美的一致性.
对于硫胺和β-乳酸胺耐药基因,发现了高度一致.
对于氨基糖化物耐药性基因,发现了适度的一致性,其中小差异与Klebsiella pneumoniae和Escherichia coli的特定单核酸多态 (SNP) 变异有关.

结论:

HS12a/MDR-FC试验显示了51个ARG的广泛覆盖,为其他试验提供了一个快速的替代方案,目标较少,尽管观察到一些虚假阴性.
该试验对快速抗菌药物耐药性监测和抗菌药物管理充满希望,特别是在宏化物,硫胺和β-乳酸盐耐药性方面.
未来的改进应该集中在扩大基因标,结合关键的耐药性突变,并检测新兴变异以提高诊断效用.

关键词:

这是一个DNA微阵列.在WGS中,使用的是WGS.抗微生物耐药性抗微生物耐药性快速微生物学快速微生物学

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