整合性基因组学和多组织转录组学确定了草地鱼中低氧耐受性的关键位置和途径
Wenwen Wang1,2, Mengyang Chang1, Suxu Tan1,2
1Institute of Aquatic Biotechnology, College of Life Sciences, Qingdao University, Qingdao 266071, China.
Animals : an open access journal from MDPI
|December 30, 2025
概括
研究人员通过全基因组关联分析和转录组分析在草中确定了低氧耐受性的遗传标记. 这项研究为选择性育种计划提供了宝贵的见解,以改善低氧条件下的鱼类生存.
科学领域:
- 水产养殖遗传学 水产养殖遗传学
- 鱼类生理学 鱼类生理学
- 环境压力反应环境压力反应
背景情况:
- 低氧显著影响水产养殖中的鱼类生存和生长.
- 了解低氧耐受性的遗传基础对于开发有弹性的鱼类种群至关重要.
研究的目的:
- 为了确定与草鱼 (Ctenopharyngodon idella) 的低氧耐受性相关的遗传位置和分子途径.
- 为选择性育种计划提供遗传标记,旨在提高低氧耐受性.
主要方法:
- 全基因组关联分析 (GWAS) 对2000只遭受低氧压力的草进行.
- 对低氧耐受 (HT) 和低氧不耐受 (HI) 个体的多组织转录组分析.
- 使用竞争性等位基特定PCR (KASP) 验证显著单核酸多态 (SNPs).
主要成果:
- GWAS确定了21个与低氧耐受性相关的SNP和6个与低氧耐受性相关的InDels,其中两个SNP达到全基因组意义.
- 转录组分析显示,HT和HI组之间的六种组织中基因表达的显著差异.
- 综合分析强调了16个共享基因,包括usf1和trpv4,可能调节缺氧耐受性.
结论:
- 关键的基因组区域和分子通路,包括类固醇生物合成和氧气运输,都参与了草的低氧耐受性.
- 已识别的遗传变异和基因为草繁殖中标记辅助选择提供了潜在的标记物.
- 这项研究有助于通过提高鱼类对低毒环境的抵抗力来改善水产养殖的可持续性.
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