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Reina Ueda¹, Yuko Kihara¹, Aki Tanaka¹

¹Laboratory of Wildlife Medicine, Department of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi 180-8602, Tokyo, Japan.

Animals : an open access journal from MDPI

|December 30, 2025

概括

法医兽医调查从结合肌肉组织采样和表面擦拭来进行动物伤口的DNA分析中受益. 这种双重方法提高了对违规动物物种的检测,提高了案件可靠性.

科学领域:

法医兽医科学法医兽医科学
分子生物学分子生物学
动物法医动物医学

背景情况:

确定动物死亡的原因,特别是那些缺失身体部位的动物死亡,需要区分人类和动物的罪犯.
对生物痕迹的DNA分析对于识别在法医兽医病例中造成伤害的动物至关重要.
关于肌肉组织采样与用于伤口DNA分析的表面擦拭的有效性,存在有限的比较研究.

研究的目的:

为了比较肌肉组织采样和表面擦拭用于线粒体DNA (mtDNA) 分析在法医兽医病例中的有效性.
识别可疑动物死亡中造成伤害的动物物种,包括伤口或缺失的身体部位.
评估两种采样方法的综合效用.

主要方法:

线粒体DNA (mtDNA) 分析是在三起法医兽医尸体解剖病例的样本上进行的.
采用了两种样本采集方法:从伤口取肌肉组织样本和伤口区域的表面擦拭.
案例包括可疑的动物死亡,有伤口或缺失的身体部位.

主要成果:

在案例1 (猫尸体) 中,使用这两种方法检测到狗DNA.
在案例2 (猫尸体) 中,只通过抽取样本检测到狗DNA.
在案例3 (子尸体) 中,只通过肌肉组织采样检测到猫的DNA.

结论:

肌肉组织采样保留了来自深层组织的DNA完整性.
表面擦拭是非侵入性的,覆盖更广的区域,作为一个补充工具.
结合肌肉组织采样和表面擦拭,提高了法医伤口分析中的DNA检测效率和可靠性.

关键词:

线粒体中的s(mt) DNA.狗咬伤伤害伤害狗咬伤伤害伤害法医兽医医学法医兽医医学肌肉组织采样肌肉组织采样标识物种标识物种标识物种抽样样本是抽样样本.

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