与北肌肉发育相关的ceRNA调控网络的构建和功能分析 白色喀什米尔山羊 白色喀什米尔山羊
Lina Liu1, Fenghong Wang2, Long Zhou1
1Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, College of Advanced Agricultural Sciences, Yulin University, Yulin 719000, China.
Animals : an open access journal from MDPI
|December 30, 2025
概括
长非编码RNAs (lncRNAs) 调节喀什米尔山羊的肌肉发育. 一种新的lncRNA,MSTRG.5182.1,被确定通过影响chi-miR-424-5p/IKBKG通路来控制肌形成.
科学领域:
- 动物科学动物科学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 长非编码RNAs (lncRNAs) 是生物过程的关键调节者,包括肌肉发育.
- 喀什米尔山羊肌体发生中的lncRNAs的特定功能在很大程度上仍未被探索.
研究的目的:
- 为了研究在喀什米尔山羊肌肉形成过程中 lncRNAs 的转录组景观.
- 为了确定参与喀什米尔山羊肌体发生的关键 lncRNA.
- 在这个过程中阐明新型lncRNA,MSTRG.5182.1的调节作用.
主要方法:
- RNA测序 (RNA-seq) 用于在四个发育阶段在长脊肌中对 lncRNAs 进行分析.
- 进行了生物信息分析,以识别和表征 lncRNAs.
- 进行了实验验证,以评估MSTRG.5182.1.的功能.
主要成果:
- 共有3480个lncRNAs被确定,其中包括1141个新型.
- 下调MSTRG.5182.1抑制了肌细胞的增殖,促进了分化.
- 击败MSTRG.5182.1通过调节chi-miR-424-5p/IKBKG信号通路来影响肌肉生长.
结论:
- 这项研究提供了在喀什米尔山羊肌肉发育过程中全面的lncRNA表达特征.
- MSTRG.5182.1通过chi-miR-424-5p/IKBKG通路在喀什米尔山羊肌体发生过程中发挥着重要作用.
- 研究结果提供了关于肌肉发育的分子机制的见解,以及在育种和改善肉质方面的潜在应用.
更多相关视频
10:13Methods to Assess Subcellular Compartments of Muscle in C. elegans
Published on: November 13, 2014
17.1K
07:46Author Spotlight: Improving Beef Cattle Nutrition and Production with a Focus on Feed Efficiency and Meat Quality Traits Through Advanced Biochemical and Molecular Assays
Published on: July 12, 2024
936
相关概念视频
01:13Formation of Muscle Fibers from Myoblasts
5.7K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.7K
02:23Master Transcription Regulators
7.6K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K
02:02Cis-regulatory Sequences
11.5K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.5K
02:02Cis-regulatory Sequences
3.9K
3.9K
01:51Determination
20.6K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
20.6K
01:23Regulation of Expression at Multiple Steps
1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K