对比转录组分析揭示了在不同的光持续时间刺激下,Poria cocos中生长活动的调节
Chengwen Wu1, Shanwen Ye2, Xuhui Wei3
1Youxi Forestry Science and Technology Promotion Center, Sanming 365100, China.
Genes
|December 30, 2025
概括
光线持续时间通过调节基因表达,显著影响Poria cocos的生长. 该研究确定了关键的蓝光反应基因 (BLI) 和参与生长和应激反应的代谢途径.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 植物生理学 植物生理学
背景情况:
- 光是影响真菌生长和新陈代谢的关键环境因素.
- Poria cocos是一种药用真菌,对不同的光持续时间表现出不同的反应.
- 了解光的调节作用对于优化种植和代谢过程至关重要.
研究的目的:
- 阐明波利亚可可斯通过不同的光持续时间来调节生长的分子机制.
- 识别关键的基因和受光照影响的信号通路.
- 确定光在 Poria cocos.中的代谢调节和应激反应中的作用.
主要方法:
- 转录组测序 (RNA-seq) 用于在不同的光疗 (0,15,30天) 下分析基因表达特征.
- 定量实时PCR (qRT-PCR) 用于对差异表达基因 (DEGs) 的验证.
- 权重基因共同表达网络分析 (WGCNA) 用于识别基因调节网络.
主要成果:
- 总共有4332个差异表达的基因被确定,蓝光反应基因 (BLI-3,BLI-4) 在15天后显著上调,随后在30天后下调.
- 凯格缩分析表明,DEGs主要参与二次代谢物合成,碳代谢,氨基酸合成,氧化还原反应和MAPK信号传递.
- 活跃的生长代谢 (CYP,SNF1,COX基因) 在15天被观察到,而氧化应激 (ROS相关基因) 在0和30天发生,影响生长. BLI基因还激活了欧戈斯特醇合成,增强了细胞膜稳定性.
结论:
- 这项研究提供了关于光线持续时间如何调节Poria cocos生长和活动的第一个系统的分子洞察.
- BLI基因家族在光信号感知和新陈代谢控制中发挥着核心作用,影响埃尔戈斯特的合成,能量代谢和氧化应激.
- 提出了一个潜在的"BLI-4-MAPKKK-CYP"监管轴,为优化Poria cocos种植和了解真菌环境反应提供理论支持.
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