林,谷氨酸,白氨酸丰富蛋白1 (PELP1):跨物种的多样性,结构保护和进化起源
Nomfundo Mngomezulu1, Siphesihle M Msweli1, Suresh B Pakala1,2
1Department of Biochemistry and Microbiology, Faculty of Science, Agriculture and Engineering, University of Zululand, Empangeni 3886, South Africa.
富含氨酸,氨酸和氨酸的蛋白质1 (PELP1) 仅在opisthokont真核生物中发现,而不是植物或细菌. 尽管有序列差异,但PELP1保持着保存的结构和进化起源,这表明功能性适应.
科学领域:
- 分子生物学分子生物学
- 进化生物学 进化生物学
- 结构生物学 结构生物学
背景情况:
- 氨酸,谷氨酸,白氨酸丰富蛋白1 (PELP1) 是核蛋白,对核糖体生物发生和类固醇受体信号传递至关重要.
- PELP1拥有RIX1和NUC域,这对rRNA处理和核细胞功能至关重要.
- 关于PELP1的进化历史和哺乳动物以外的结构多样性的知识有限.
研究的目的:
- 研究PELP1蛋白质在各种生命形式中的分布,结构特征和进化起源.
- 识别PELP1蛋白内保存域和动图,并分析它们的氨基酸组成.
- 阐明PELP1.1的进化轨迹和植物遗传关系.
主要方法:
- 使用NCBI,UniProt和EukProt数据库对PELP1蛋白质进行全面的数据挖掘.
- 域和图案分析,包括LXXLL和PXXP图案.
- 结构建模和植物遗传学分析,以评估保护和进化历史.
主要成果:
- PELP1蛋白质仅在Opisthokonta类 (真菌,菲拉斯特雷亚,甲基动物) 中的真核生物中被确定.
- PELP1蛋白质含有RIX1和NUC202域,尽管具有低序列相同性,但具有保留的α-螺旋结构.
- 进化分析表明PELP1起源于opisthokont分歧之前,已经确定了三种不同的血统.
结论:
- PELP1蛋白质仅限于opisthokonts,表现出显著的序列分歧,但保留了结构结构.
- 保存的结构表明进化适应,保持PELP1的功能完整性.
- PELP1很可能是从一种含有RIX1的蛋白质进化而来的,该蛋白质获得了NUC202域,早于opisthokont的多样化.
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