相关实验视频
Updated: Jan 7, 2026

10:36
Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
14.8K
碳酸无水酶3过度表达调节与细胞应激弹性和蛋白质稳定相关的信号通路
Yezhou Yu1,2, Merrina Anugraham2, Tony Blick3
1School of Environment and Science, Griffith University, Brisbane, QLD 4111, Australia.
International journal of molecular sciences
|December 30, 2025
概括
碳酸无水酶3 (CA3),尽管酶活性较低,但在细胞保护中起着至关重要的作用. 过度表达CA3会影响蛋白质合成和信号通路,这表明它在应激弹性方面起着更广泛的作用.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 碳酸无水酶3 (CA3) 具有较低的酶活性,但具有独特的表面囊蛋白,易受氧化应激的影响.
- CA3在肌肉中表达很高,并且与细胞保护有关,特别是通过与BAG3的相互作用和自的调节.
- 以前的研究表明,CA3的过度表达可以保护心肌细胞免受缺氧诱导的亡.
研究的目的:
- 研究CA3过度表达对细胞通路和蛋白质相互作用的影响.
- 确定新的CA3结合伙伴,并了解CA3的非酶功能.
- 探索CA3在细胞应激反应机制中的作用.
主要方法:
- 用RNA测序和蛋白质组学来分析HEK293T,MDA-MB-231和SVCT细胞中的细胞变化.
- 对RNA测序数据进行了基因组丰富分析 (GSEA).
- 用HaloTag拉下实验,然后进行质谱测量,用于蛋白质组分析.
主要成果:
- 在HEK293T细胞中,CA3过度表达导致了参与蛋白质合成,RNA处理和神经退行性疾病信号传递的通路的下调.
- 在乳腺上皮细胞中观察到类似的通路下调,缺氧额外抑制了干扰素信号传递.
- 蛋白质组学确定了热冲击蛋白和核糖体蛋白S2作为潜在的CA3结合伙伴,RANBP2被持续上调.
结论:
- CA3调节关键的细胞过程,包括蛋白质合成和信号,独立于它的酶活性.
- 通过途径调节和蛋白质相互作用,CA3有助于细胞应激弹性.
- 研究结果表明,CA3在自和神经退行性疾病途径中的参与需要进一步调查.
相关概念视频
mTOR Signaling and Cancer Progression
4.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.6K
Other Stress Responses in Bacteria
308
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
308
Interactions Between Signaling Pathways
7.1K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.1K
Regulation of Angiogenesis and Blood Supply
3.3K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.3K
Regulation of the Unfolded Protein Response
2.9K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.9K
Renal Regulation of Acid-Base Balance
1.5K
Metabolic reactions in the body produce nonvolatile acids, such as sulfuric acid, which generate an acid load of approximately 1 mEq of H+ per kilogram of body weight daily. Excreting H+ in the urine is essential to balance this acid load.
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
1.5K

