淡水克拉多鱼的内分泌干扰:对TBOEP和PFECHS的转录组网络视角 影响大的Daphnia magna
相关实验视频
Updated: Jan 7, 2026
09:43
Methods to Test Endocrine Disruption in Drosophila melanogaster
Published on: July 3, 2019
10.2K
Hyun Woo Kim1, Seok-Gyu Yun2, Ju Yeon Park1
1Department of Life Science, Biomedi Campus, Institute of Environmental Medicine for Green Chemistry, Dongguk University, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Republic of Korea.
International journal of molecular sciences
|December 30, 2025
概括
两个阻燃剂,TBOEP和PFECHS,破坏了大的内分泌网络,影响了生长和繁殖,但没有导致死亡. 枢纽基因和Wnt信号通路被确定为监测混合内分泌干扰化学物质暴露的潜在生物标志物.
科学领域:
- 环境毒理学环境毒理学
- 分子生物学分子生物学
- 生态毒理学 生态毒理学
背景情况:
- 大是内分泌干扰化学物质 (EDC) 的敏感指标,由于荷尔蒙调节的生命史特征.
- 阻燃剂三二乙烯酸盐 (TBOEP) 和 perfluoroethylcyclohexane sulfonate (PFECHS) 是地表水中同时存在的污染物.
- 这些同时发生的EDC对无脊椎动物内分泌系统的慢性亚致命作用基本上是未知的.
研究的目的:
- 调查TBOEP和PFECHS对大夫尼亚大的内分泌网络的亚致命影响.
- 为暴露于TBOEP和PFECHS的Daphnia magna建立假定的不良结果途径 (AOP).
- 为了确定监测混合EDC暴露的潜在生物标志物.
主要方法:
- 分析了两个公开可用的21天微阵列数据集 (TBOEP: GSE55132; PFECHS: GSE75607).
- 基因本体学丰富的应用,STRING蛋白相互作用网络,Drosophila表型映射和KEGG定框架.
- 将差异性表达的基因分组为功能模块,并根据程度和间距对枢纽节点进行排序.
主要成果:
- 暴露于TBOEP抑制了变形和生长,影响了1157个与代谢和膜过程相关的基因,这些基因是肌肉发育的关键枢纽.
- 暴露在PFECHS下调了维特洛根因和改变了879个与氧化应激和谷氨代谢相关的基因,其中中心节点参与了 oogenesis.
- 无论是TBOEP还是PFECHS都集中在Wnt信号通路上,减少了TCF/LEF依赖的转录,预测了受损的卵细胞成熟,降低了生育能力和发育迟缓.
结论:
- 网络分析显示,TBOEP和PFECHS破坏了Daphnia magna的内分泌,发育和代谢途径的稳定性,而不会导致致命性.
- Wnt信号通路的关键事件和已识别的枢纽基因作为混合EDC暴露的敏感生物标志物.
- 未来需要低剂量研究,以环境现实的场景来评估这些候选生物标志物.
相关概念视频
02:27Background and Environment Affect Phenotype
7.3K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.3K
02:32Osmoregulation in Fishes
52.6K
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
52.6K