病原体的鉴定和治疗Trichoderma koningiopsis ZL01火中的菌Pygoluciola sp. (Coleoptera:Lampyridae) 是一个类型的类动物
Yan-Hong Chen1, Shi-Ling Wang1, Fu-Xin Li1
1Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
Insects
|December 30, 2025
概括
一种新的真菌,Trichoderma koningiopsis,在人工繁殖期间在火虫幼虫中引起致命的真菌菌. 抗真菌药物尼斯塔丁有效治疗这种感染,显著增加幼虫存活率.
科学领域:
- 兽医真菌学 兽医真菌学
- 昆虫病理学 昆虫病理学
- 保护生物学 保护生物学
背景情况:
- 人工繁殖对于火虫种群的支持至关重要.
- 菌病爆发在被囚禁的火虫幼虫中越来越常见,导致高死亡率.
- 对于可持续的火虫繁殖计划,需要病原体识别和治疗策略.
研究的目的:
- 为了确定负责菌的微真菌在幼虫中Pygoluciola sp. 在人工繁殖期间.
- 评估潜在的抗真菌剂对已识别的真菌的有效性和安全性.
- 评估最佳抗真菌治疗对幼虫存活率的影响.
主要方法:
- 使用形态和分子分析来识别引起的真菌病原体.
- 选了五种抗真菌剂,并确定了它们对真菌的最小抑制度 (MIC).
- 在体内对Pygoluciola sp.进行了毒性测试. 幼虫来评估抗真菌候选物的安全性.
- 尼斯塔丁的疗效通过比较通过喷雾和滴水管理治疗的感染幼虫的生存率与未经治疗的对照进行了评估.
主要成果:
- 这种微真菌被确定为Trichoderma koningiopsis (ZL01菌株).
- 尼斯塔丁对T. koningiopsis ZL01表现出最佳的抗真菌活性,MIC为25.00μg/mL.
- 尼斯塔丁被发现对Pygoluciola sp.是安全的 通过急性口服和接触性毒性测试来测试幼虫.
- 尼斯塔丁治疗导致喷雾剂的存活率增加了两倍,滴水剂的存活率增加了三倍,持续了九天.
结论:
- 三虫 (Trichoderma koningiopsis) 被确定为一种致病原体,在半水生火幼虫中引起菌根.
- 尼斯塔丁是一种安全有效的抗真菌剂,用于控制人工繁殖的Pygoluciola sp.中的T. koningiopsis感染.
- 这项研究为半水生虫的病原体感染提供了第一个文件,并提供了一种可行的治疗策略,这对保护工作至关重要.
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