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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jan 7, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

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测绘RNA修饰量的定量技术的进步

Ling Tian1, Bharathi Vallabhaneni1, Yie-Hwa Chang2

  • 1Mediomics, LLC, 5445 Highland Park Drive, Saint Louis, MO 63110, USA.

Life (Basel, Switzerland)
|December 30, 2025
PubMed
概括
此摘要是机器生成的。

RNA的修改调节了基因表达和细胞功能. 本综述详细介绍了分析这些关键RNA修饰的方法,有助于发现疾病生物标志物和治疗开发.

关键词:
基因组RNA的修改 基因组RNA的改变生物标志物发现发现通过化学辅助测序进行测序.标志性转录组学 标志性转录组学质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量纳米孔直接RNA测序单细胞分析 单细胞分析单个分子成像技术

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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
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相关实验视频

Last Updated: Jan 7, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物化学 生物化学

背景情况:

  • RNA的修改是基因表达和细胞功能的重要调节者,影响RNA的稳定性,拼接,翻译和定位.
  • 它们的失调与各种疾病有关,包括癌症,神经退行性疾病和病毒感染.
  • 准确量化和绘制RNA修饰的地图对于阐明它们的生物学作用至关重要.

研究的目的:

  • 审查和总结分析RNA修饰的当前和新兴方法.
  • 讨论每个分析技术的优点,局限性和挑战.
  • 为选择适当的RNA修饰分析策略提供一个框架.

主要方法:

  • 质谱测量质量谱测量
  • 基于抗体和非抗体的检测方法.
  • 基于PCR和NMR的检测检测
  • 化学和酶辅助的测序.
  • 纳米孔直接RNA测序
  • 单细胞和单分子成像技术.

主要成果:

  • 介绍了各种RNA修饰分析技术的全面概述.
  • 该审查批判性地评估了每个方法的优点和弱点.
  • 突出了单细胞和单分子分析的先进技术.

结论:

  • 为表表转录组分析提供了一个强大的工具包,涵盖了各种技术.
  • 这些方法对于理解RNA修饰功能和识别疾病生物标志物至关重要.
  • 未来的研究应该专注于高通量,多重复合和单细胞方法,以实现全面的表表体转录组解码.