脱电喷雾电离化质谱成像:原理,进展和多学科应用
Yan Wang1,2, Huannan Wang2, Shuncun Zhang2
1School of Pharmaceutical Sciences & Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China.
Journal of mass spectrometry : JMS
|December 30, 2025
概括
脱电喷雾电离-质谱成像 (DESI-MSI) 提供了对生物组织的无标签分子映射. 尽管存在解决挑战,但进步提高了其在生物医学,制药和植物科学中的应用.
科学领域:
- 分析化学 分析化学
- 生物医学成像技术 生物医学成像技术
- 质谱测量质量谱测量
背景情况:
- 像DESI-MSI这样的环境电离技术可以在没有复杂的样本准备的情况下进行分子映射.
- 自2004年以来,DESI-MSI在仪器仪表和数据处理方面取得了进步,用于可视化组织中的分子分布.
- 它在大气压下提供无标签,无矩阵的分析.
研究的目的:
- 系统地审查DESI-MSI的原则,仪器和参数.
- 评估最近的技术进步,包括纳米级的DESI和增强的溶剂添加剂.
- 检查DESI-MSI在各种科学领域的多学科应用.
主要方法:
- 审查DESI-MSI的基本原则和仪器配置.
- 对技术进步的批判性评估,如纳米级的DESI和添加剂增强溶剂.
- 综合检查生物医学,制药和植物科学中的多学科应用.
主要成果:
- DESI-MSI提供了组织中各种分子 (脂质,代谢物,药物) 的高通量可视化.
- 纳米级的DESI实现了50微米以下的空间分辨率;添加剂增强型溶剂提高了灵敏度和特异性.
- 应用包括癌症边缘划分,生物标志物发现,药物分布分析和植物化学映射.
结论:
- DESI-MSI是空间代谢学的一个变革性工具,在临床诊断和药物查方面具有潜力.
- 未来的方向包括多式联络成像,用于数据分析的机器学习和临床翻译.
- 尽管有优势,但空间分辨率和数据解释方面的挑战仍然存在.
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