相关实验视频
Updated: Jan 7, 2026

13:00
Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
2.7K
由表面脂蛋白结合的核酸结合控制了在真菌质体中的结合性ICE获取
M'hamed Derriche1, Laurent Xavier Nouvel1, Calvin Fauvet1
1IHAP, Université de Toulouse, INRAE, ENVT, Toulouse, France.
mBio
|December 30, 2025
概括
一种表面脂蛋白,P48,对于细菌来说至关重要,以获得称为整合性和结合性元素 (ICE) 的移动DNA元素. 这一发现凸显了接受细胞在基因转移中的重要性,并提出了控制细菌传播的新方法.
科学领域:
- 微生物学 微生物学
- 细菌遗传学 细菌遗传学
- 横向基因转移是指水平基因转移.
背景情况:
- 整合性和结合性元素 (ICE) 是细菌水平基因转移 (HGT) 的关键驱动因素,促进基因交换.
- 细菌结合的研究在历史上一直集中在供体细胞和ICE机制上,很大程度上忽视了受体细胞在基因获取中的作用.
- 了解接收因素对于全面了解HGT动态和制定控制它的策略至关重要.
研究的目的:
- 识别受体细胞因子,这些因子对于细菌中集成和结合元件 (ICE) 的获取至关重要.
- 阐明受体细胞促进ICE吸收的机制.
- 通过突出受体介导的过程,挑战以供体为中心的细菌结合范式.
主要方法:
- 利用全基因组的转子子突变发生在 * Mycoplasma agalactiae * 和 * Mycoplasma bovis * 中,以确定对 ICE 获取至关重要的基因.
- 雇佣高通量交配测试以选与ICE吸收有关的受体因素.
- 对已识别的因素进行结构和功能分析,包括点突变和传送器失活,以确定它们在结合中的作用.
主要成果:
- 表面脂蛋白P48被确定为*M. agalactiae*和*M. bovis*中ICE吸收的主要决定因素.
- P48作为ABC输送器的基质结合元件,核酸结合对于ICE获取至关重要,但不是核酸运输本身.
- 失去P48诱导了遗留ICE基因的转录激活,表明表面识别影响受体的细胞内状态,而P48的作用是获得特异性的.
结论:
- 一个以前未被识别的,表面暴露的受体因子 (P48) 对于菌质体中高效的ICE转移至关重要.
- 核酸结合,而不是运输,是细菌结合的中心功能,将营养感应与基因获取联系起来.
- 这项工作将结合的范式转变为共同决定的过程,并建议新的策略来限制致病性和合成细菌中的HGT.
相关概念视频
Conjugated Proteins
27.0K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
27.0K
Formation of Lipopolysaccharides
481
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
481
Receptor-mediated Endocytosis
110.2K
Overview
110.2K
Receptor-mediated Endocytosis
7.4K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
7.4K
Mechanism of Conjugation
710
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
710
Regulation of Nuclear Protein Sorting
3.1K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.1K

