皮肤模板支持表皮再生和成熟,无论细胞捐赠者的年龄如何
Autumn C Campbell1, Divya Sridharan2,3, Britani N Blackstone1
1Department of Materials Science and Engineering, The Ohio State University, Columbus, Ohio, USA.
Tissue engineering. Part A
|December 30, 2025
概括
成人纤维细胞可以用于创建皮肤模板 (DTs) 创伤愈合,显示与新生儿纤维细胞相似的结果,但收缩较少. 这一发现可能允许在再生医学中治疗更大的伤口区域.
科学领域:
- 再生医学是一种再生医学.
- 组织工程是组织工程.
- 皮肤病学 皮肤病学
背景情况:
- 全厚度的烧伤会破坏皮肤,需要皮肤模板 (DTs) 来进行表皮再生.
- 新生儿前皮纤维细胞传统上用于DTs,因为假设的优越的增殖和愈合能力.
- 这项研究调查了成人纤维细胞与新生儿纤维细胞在DT构建和伤口愈合方面的疗效.
研究的目的:
- 为了比较成人和新生儿纤维细胞在皮肤模板结构中的功能.
- 评估纤维细胞源对工程皮肤表皮再生的影响.
- 评估成年纤维细胞治疗较大的伤口区域的潜力.
主要方法:
- 从新生儿和成人手术丢弃组织中分离出初级纤维细胞.
- 基因表达 (COL1A1,MMP1,MMP3,MMP9) 和基因增殖在二维和三维培养中进行了评估.
- 使用电式原体支架构建DTs,并在7天内评估它们的收缩,矩阵重塑和细胞活力.
- 用工程皮肤结构播种了角质细胞,以评估表皮再生.
主要成果:
- 在2D培养中,新生儿和成人纤维细胞之间没有观察到基因表达的显著差异.
- 新生儿纤维细胞在2D中表现出更高的增殖,但在3DDDTs中增殖是独立于年龄的.
- 新生儿DT的收缩幅度明显高于成人DT (91.7%vs68.8%).
- 这两种DT类型都支持强大的表皮再生,在皮肤/表皮厚度,角质细胞增殖,屏障功能或底层膜沉积方面没有显著差异.
- 在工程皮肤中,适度的基因表达差异 (MMP1, COL1A1, ACTA2) 与组织学变异无关.
结论:
- 成人纤维细胞适合皮肤模板制造,促进表皮再生,与新生儿纤维细胞相似.
- 用成年纤维细胞进行的DT显示了体外收缩的减少,这可能使更大的伤口区域能够得到治疗.
- 成人纤维细胞的使用为再生医学在烧伤治疗和伤口愈合中的应用提供了一个可行的替代方案.
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