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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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相关实验视频

Updated: Jan 7, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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CircTarget:一个全面的数据库,包含跨多种细胞类型的circRNA-目标RNA相互作用.

Hongmei Zhang1,2,3, Yuyang Zhang4, Peng Li2

  • 1Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510100, China.

Nucleic acids research
|December 30, 2025
PubMed
概括

循环RNAs (circRNAs) 是关键的基因调节剂,但它们的点是未知的. CircTarget是一个新的数据库,以高分辨率映射成千上万个经过实验验证的circRNA-目标RNA相互作用.

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相关实验视频

Last Updated: Jan 7, 2026

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 循环RNAs (circRNAs) 是普遍存在的调节分子,其来源于mRNA前的逆拼接.
  • circRNAs在基因调节和疾病发展中起着至关重要的作用.
  • 识别circRNA目标是必不可少的,但仍然是该领域的一个重大挑战.

研究的目的:

  • 开发第一个实验验证的circRNA-目标RNA相互作用的全面数据库.
  • 提供这些相互作用的单核酸分辨率映射.
  • 促进研究circRNA功能和疾病机制.

主要方法:

  • 来自各种交叉连接方法的RNA-RNA交互原子数据的分析.
  • 来自13个细胞系和人类/小鼠海马组织的数据集成.
  • 使用严格的标准识别高可信度circRNA-目标RNA相互作用.

主要成果:

  • 发现了132,517个高度可靠的circRNA-目标RNA相互作用.
  • 开发CircTarget,这是一个数据库,每个交互都有详细的注释.
  • 包括circRNA信息,疾病关联,相互作用部位和相关的遗传变异.

结论:

  • CircTarget是探索circRNA功能和相互作用的宝贵资源.
  • 该数据库提供了关于circRNA-目标RNA结合的实验支持的证据.
  • 通过CircTarget,可以更深入地研究circRNAs在基因调节和疾病发病过程中的作用.