评估上呼吸道采样对囊性纤维化病微生物监测的可靠性
Gisli G Einarsson1, Andrew J Lee1, Peter J Barry2
1School of Pharmacy. Queen's University Belfast, Belfast United Kingdom, BT9 7BL.
概括
在囊性纤维化 (CF) 患者中,上呼吸道冲洗样品不能可靠地检测下呼吸道病原体. 然而,上呼吸道微生物群可能反映了CF中肺异位症的整体程度.
科学领域:
- 微生物学 微生物学
- 肺部病理学 肺部病理学
- 遗传学 遗传学 是一个
背景情况:
- 唾液分析是囊性纤维化 (CF) 微生物监测的标准.
- CFTR调节器疗法减少了唾液,需要使用替代的采样方法.
- 上呼吸道采样是一种潜在的替代品,但其下呼吸道代表性尚不清楚.
研究的目的:
- 评估上空气道微生物群的概况是否可靠地代表了CF成年人的下空气道微生物群.
- 为了比较口腔喉冲洗 (OPR) 和液微生物群在CF患者和健康志愿者.
主要方法:
- 对70名患有CF的成人和10名健康志愿者的前性观察研究.
- 16S rRNA OPR和唾液样本的基因测序.
- 分析alpha和beta多样性,分类组合,以及与健康对照的差异.
主要成果:
- 在CF患者中,OPR和唾液微生物群的组成是不同的;健康的志愿者显示了类似的社区.
- 坚果菌在OPR中占主导地位,而蛋白质菌 (伪菌) 在唾液中占主导地位.
- 在这两种样本类型中,随着肺功能恶化,失生症的增加,但上/下呼吸道分歧与肺功能无关.
结论:
- OPR采样不能可靠地识别CF的关键下呼吸道病原体.
- 上呼吸道采样可能反映了CF患者下呼吸道失调的程度.
- 进一步调查上呼吸道采样作为CF肺部健康的替代品是有必要的.
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