香烟烟雾破坏G-四重复结构的稳定,并对抗巨细胞中的G4-连接体效应
Davida Mirra1, Erica Salvati2, Renata Esposito1
1Department of Environmental Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, Italy.
Scientific reports
|December 30, 2025
概括
暴露在香烟烟雾中会破坏巨细胞中的G-四重复 (G4) 结构,影响基因表达,并可能导致吸烟相关的疾病和癌症. 这项研究揭示了癌症发生过程中一种新的G4依赖机制.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 癌症研究 癌症研究
背景情况:
- 香烟烟雾是呼吸系统疾病和癌症的重要危险因素.
- 巨细胞是受CS影响的关键免疫细胞,影响瘤促进.
- G四重复体 (G4) 是非正规的核酸结构,调节基因表达和DNA稳定性.
研究的目的:
- 研究卷烟烟雾暴露对巨细胞G-四重复合体 (G4) 形成和稳定性的影响.
- 探索潜在的G4依赖机制,是CS诱导的炎症和基因表达变化的基础.
- 为了确定CS暴露是否影响G4配体的抗增殖作用.
主要方法:
- 培养的THP-1单细胞分化为巨细胞被暴露在香烟烟雾中.
- 评估了在存在或不存在CS和G4连接体RHPS4.4的情况下G4结构形成和稳定性.
- 分析了包括IL-1β,TNF-α,myc和bcl2在内的关键基因的表达水平.
主要成果:
- CS暴露破坏了巨细胞中的G4结构的稳定性,抵消了RHPS4.4的稳定作用.
- CS显著诱导IL-1β和TNF-α的激活,这些细胞因子在其促进体中具有假定的G4区域.
- CS对抗RHPS4的抗增殖作用,并改变了myc和bcl2.2等G4含有瘤基因的表达.
结论:
- 暴露于CS会破坏巨细胞中的G4结构,这代表了吸烟相关病理的一个新机制.
- G4依赖调节可能是CS诱导的细胞因子调节的基础,并影响癌症发生.
- 针对G4结构可以为吸烟相关疾病和癌症提供新的治疗策略.
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