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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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相关实验视频

Updated: Jan 7, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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TUG1:一种潜在的内源性参考基因,用于血液研究中的长非编码RNA量化.

Carlos Rodríguez-Muñoz1,2, Anna Vila1, Sally Santisteve1,2

  • 1Translational Research in Respiratory Medicine, University Hospital Arnau de Vilanova and Santa Maria, IRBLleida, Avda, Alcalde Rovira Roure 80, Lleida, 25198, Spain.

Biomarker research
|December 30, 2025
PubMed
概括

确定稳定的长非编码RNA (lncRNA) 控制对准确的基因表达分析至关重要. 这项研究发现,TUG1是最稳定的lncRNA,用于在全血样本中规范定量PCR数据.

关键词:
内源性控制是一种内源性控制.长长的非编码RNAs.规范化 规范化 规范化周围血液 周围血液实时聚合酶连锁反应的定量实时反应.参考基因是指基因中的基因.稳定的稳定性 稳定的稳定性在TUG1上,我们将在TUG1上工作.这是全血,全血.

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相关实验视频

Last Updated: Jan 7, 2026

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物标志物发现发现

背景情况:

  • 准确量化长非编码RNAs (lncRNAs) 对于它们作为生物标记物的使用至关重要.
  • 定量PCR (qPCR) 需要稳定的内源控制来实现可靠的基因表达正常化.
  • 在全血样本中识别合适的参考基因具有挑战性.

研究的目的:

  • 为了确定稳定的长非编码RNA (lncRNA) 参考基因在全血样本中进行正常化.
  • 用已确定的算法评估候选 lncRNAs 的稳定性.
  • 使用 lncRNA 和信使RNA (mRNA) 的不同规范化策略的疗效进行比较.

主要方法:

  • 在182个全血样本中,RT-qPCR分析了84个lncRNA和8个mRNA参考基因.
  • 使用geNorm,NormFinder和BestKeeper算法对转录稳定性的评估.
  • 对平均中心化和RNA测序数据集的规范化策略的评估.

主要成果:

  • 29个lncRNA符合表达标准,TUG1始终被确定为所有算法中最稳定的候选者.
  • TUG1规范化实现了表达变化率的降低,与平均中心化相比,优于其他策略.
  • 在外部RNA测序数据中,TUG1与临床变量存在很小的关联,并且在外部RNA测序数据中具有一致的高表达/低变化.

结论:

  • TUG1是全血样本中 lncRNA量化最稳定的内源性对照.
  • 在临床研究中,TUG1为准确的lncRNA规范化提供了一个有希望的,具有成本效益的解决方案.
  • 这一发现支持TUG1在使用全血样本的生物标志物研究中的实用性.