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人类GV卵细胞和累积细胞之间的相互相互作用:对GVBD,ROS生产和AMPK表达的影响
Zhaoqiao Ban1, Plamen Todorov2, Gohar Rahimi1,3
1Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.
Medicina (Kaunas, Lithuania)
|December 31, 2025
概括
卵细胞的成熟取决于累积细胞 (CCs),但卵细胞也会影响CCs. 共同培养表明卵细胞改善了CC活力和功能,突出显示了生殖生物学中的双向通信.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 卵细胞成熟的过程
背景情况:
- 累积细胞 (CCs) 的质量对生殖囊泡 (GV) 卵细胞成熟至关重要.
- GV卵细胞对累积细胞功能的相互影响尚不清楚.
研究的目的:
- 调查GV卵细胞和CCs之间的双向通信.
- 为了确定卵子细胞对累积细胞功能和活性的影响.
主要方法:
- GV卵细胞与或没有CCs培养.
- 累积细胞单独培养或与卵细胞一起培养.
- 评估的卵细胞成熟率 (GVBD),CC形态,生长,细胞内活性氧物种 (ROS) 和AMP激活蛋白激酶 (AMPK) 表达.
主要成果:
- 当与CCs共同培养时,卵细胞成熟率 (GVBD) 显着更高.
- 与卵细胞共同培养的CCs显示了改善的形态,细胞连接和细胞外矩阵形成.
- 与单独培养的CC相比,共同培养的CC降低了ROS水平并增加了AMPK表达.
结论:
- 累积细胞支持卵细胞成熟.
- 卵子对累积细胞的活力和功能产生互惠的有益影响.
- 卵细胞 - 积细胞相互作用是双向的,对繁殖成功至关重要.
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