相关实验视频

Updated: Jan 7, 2026

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

在番茄果实中对过敏性蛋白质编码Lyc e1的CRISPR/Cas9向基因编辑

Fanzhuang Yan¹, Jian Yao¹, Myungjin Lee²

¹Department of Smart Agriculture Systems, Chungnam National University, Daejeon 34134, Republic of Korea.

Plants (Basel, Switzerland)

|December 31, 2025

概括

科学家们使用CRISPR/Cas9基因编辑来消除番茄中的主要过敏原. 食物过敏研究中的这一突破成功地减少了蛋白蛋白,为低过敏番茄品种铺平了道路.

科学领域:

植物生物技术植物生物技术
食物过敏研究食物过敏研究
分子遗传学分子遗传学

背景情况:

番茄 (Solanum lycopersicum L.) 是全球消费的作物,也是食物过敏原的重要来源.
主要的西红过敏原 - - Profilin是由Lyc e1.01和Lyc e1.02基因编码的.
以往使用基因组编辑来消除profilin的尝试都没有成功.

研究的目的:

开发一种精确的基因组编辑策略,以完全消除西红中的蛋白质.
通过破坏Lyc e1基因来产生低过敏性番茄品种.

主要方法:

设计了一个单导向RNA,针对Lyc e1.01和Lyc e1.02.的保护区域.
在番茄中使用CRISPR/Cas9系统检测功能丧失突变.
利用Agrobacterium介导的转化来产生转基因线条,并通过基因型定型和西方涂料确认编辑.

主要成果:

在转基因番茄线的两个目标位点取得了成功的编辑.
在突变的番茄果实中显著降低了林积累.
鉴定了同卵性Cas9无线,具有显著降低的林水平.

结论:

在CRISPR/Cas9中介的Lyc e1基因中断后,可以有效地从番茄果实中去除profilin.
这一战略为开发低过敏性番茄品种提供了一个有希望的方法.
该方法有可能扩展到其他过敏原作物物种.

关键词:

通过农业细菌介导的转化过程.这就是CRISPR/Cas9的作用.没有Cas9的.个人资料个人资料番茄过敏番茄过敏

更多相关视频

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Published on: June 23, 2023

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

相关概念视频

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...