相关实验视频

Updated: May 5, 2026

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Published on: May 2, 2016

金混合纳米颗粒:用于细胞贩运多模式成像的多功能工具

Andrea Bezze¹, Jessica Ponti², Deborah Stanco²

¹Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca Degli Abruzzi 24, 10129 Torino, Italy.

|December 31, 2025

概括

研究人员开发了新的黄金合纳米粒子,以改善纳米医学中的跟踪. 这种多式成像方法增强了细胞内纳米粒子输送的可视化和量化,克服了传统方法的局限性.

科学领域:

纳米医学是一种纳米医学.
材料科学材料科学材料科学
生物技术是生物技术.

背景情况:

纳米医学为药物输送提供了希望,但在实时跟踪有机纳米粒子 (NP) 方面面临挑战.
有机NP的有限光学对比度阻碍了无标签的高分辨率成像和对NP命运的理解.
金属兴奋剂是一种使用多种成像技术同时检测的策略.

研究的目的:

开发用于多式成像和跟踪的新型金混合纳米粒子 (NP).
为了实现对生物系统中NP贩运的增强可视化和量化.

主要方法:

通过纳米沉合成的黄金合的NP,以DLS,NTA,UV/Vis光谱和TEM为特征.
评估了质母细胞瘤和微细胞中的细胞相容性和细胞吸收.
使用全息图和光共聚焦显微镜进行实时跟踪.

主要成果:

黄金注的NP表现出最佳的尺寸,稳定性和~50%的效率.
兴奋剂略微增加了NP大小和zeta潜力,但保持了细胞相容性和细胞吸收.
通过全息学实现了NP积累的无标签实时可视化,并通过光显微镜证实了这一点.
由于黄金的高电子密度,TEM促进了精确的细胞内定位.

结论:

黄金合的NP作为多式联络成像的多功能平台.
证明了非侵入性,高分辨率跟踪细胞内NP积累的潜力.
通过使用多种分析技术,方便准确量化.

关键词:

毒品贩运,贩卖细胞的行为.电子显微镜的电子显微镜黄金NP的NP是什么意思整体图形 (holotomography) 是一种全息图形.混合纳米粒子的混合纳米粒子.多模式成像技术多模式成像技术

更多相关视频

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Published on: January 15, 2018

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

相关概念视频

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.