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针对多种植物病原体的生物控制潜力,对Bacillus velezensis AN6进行隔离和基因组基因表征

Liping Yang^1,2, Anyu Gu¹, Wei Deng¹

¹Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS), Kunming 650205, China.

|December 31, 2025

概括

一种新型细菌,Bacillus velezensis AN6,可以有效控制米细菌瘟疫等植物疾病. 这种生物控制剂对病原体具有广泛的活性,并产生有益的化合物,为疾病管理提供环保的解决方案.

科学领域:

农业科学农业科学
微生物学微生物学
植物病理学植物病理学

背景情况:

生物控制为管理植物疾病提供了对化学农药的环保替代方案.
有效的生物控制剂对于可持续农业和综合性害虫防治战略至关重要.

研究的目的:

隔离和描述一种具有针对植物病原体的广谱活性的新型对抗性细菌.
识别和评估分离菌株的生物控制潜力,被指定为AN6,用于控制大米细菌病.

主要方法:

从大米植物中分离和选对抗性细菌.
在体外测定包括牛津杯,双培养,VOC和CFS测定.
使用形态和分子方法进行分类学识别 (16S rRNA, gyrA, ANI, isDDH).
全基因组测序和生物合成基因集群 (BGCs) 的分析.
实验评估生物控制对抗Xanthomonas oryzae pv.的疗效. 一个叫做Oryzae的.

主要成果:

菌株AN6显示显著抑制了Xanthomonas oryzae pv. 菌 (73.60%) 和各种致病菌.
AN6产生蛋白酶和 siderophores,有助于其对抗性质.
在分类学分析中,AN6被确定为Bacillus velezensis.
基因组测序揭示了13个BGC用于二次代谢物生产.
子实验显示,大米的细菌病变减少了80.49%.

结论:

百菌 velezensis AN6是一种强大的,广泛的生物控制剂.
这种菌株能够产生对抗性化合物,对抗主要的米病原体的有效性使其成为生物控制的有希望的候选者.
AN6可用于各种植物疾病的综合管理,为可持续农业做出贡献.

关键词:

这种病原体是Bacillus velezensis.克桑托莫纳斯里扎 (Xanthomonas oryzae pv.) 是一个植物. 奥里泽 (Xoo) 的一个名字生物控制生物控制广泛的对抗性活动.植物病原体植物病原体进行全基因组测序.

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