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Let-7f-5p通过调节受感染细胞中的脂质代谢重编程来抑制PRRSV复制

Dongfeng Jiang^1,2, Jin Huang¹, Xiaotong Wang¹

¹College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.

Veterinary sciences

|December 31, 2025

概括

猪生殖和呼吸系统综合征病毒 (PRRSV) 操纵宿主脂质代谢进行复制. 微RNA let-7f-5p通过减少脂质积累和抑制病毒蛋白表达来抑制PRRSV,提供一种潜在的抗病毒策略.

科学领域:

病毒学病毒学
分子生物学分子生物学
生物化学生物化学

背景情况:

脂质对于病毒复制和感染至关重要.
脂质代谢和PRRSV扩散之间的相互作用尚未得到充分理解.

研究的目的:

研究PRRSV感染对宿主脂质代谢的影响.
探索let-7f-5p在调节脂质代谢和PRRSV复制中的作用.

主要方法:

转录组学,脂组学,BODIPY染色,西部斑点 (WB) 和定量逆转录PCR (qRT-PCR). 转录组学,脂组学,BODIPY染色,西部斑点 (WB),以及定量逆转录PCR (qRT-PCR).
分析PRRSV感染细胞中与脂质代谢相关的基因和代谢物.
研究let-7f-5p对脂质滴和病毒蛋白质表达的影响.

主要成果:

PRRSV感染改变了脂质代谢基因和代谢物,增加了细胞内脂质滴,并上调了SREBP2.
Let-7f-5p减少了脂质滴积累,并抑制了PRRSV N蛋白质的表达.
十五种上调的脂类物种被let-7f-5p降调,影响了脂胆和脂肪酸代谢途径.

结论:

PRRSV劫持宿主脂质代谢以促进病毒复制.
Let-7f-5p通过双重机制对PRRSV表现出抗病毒作用,向脂质代谢和病毒蛋白质合成.
调节宿主脂质代谢是一种潜在的宿主导的抗病毒策略,可以对抗PRRSV.

关键词:

PRRSVV 在这个问题上.在SREBP2中,您可以使用SREBP2.让-7f-5p成为-7f-5p.脂质新陈代谢脂质新陈代谢猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪

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