托尔 (拉巴胺的目标) 活动的节律性支持神经状体中循环功能
Golnoush Akhtari1, Milad Falahat Chian1, Ethan Myles Sooklal1
1Department of Biology, York University, Toronto, ON, Canada.
Journal of biological rhythms
|December 31, 2025
概括
拉巴胺素的目标 (TOR) 途径显示昼夜节律性,这对于维持细胞节律至关重要,即使没有转录/翻译反循环 (TTFLs). 这一发现强调了TOR作为昼夜系统的关键调节者.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 循环节律在真核生物中是基本的,但它的生成和调节,特别是非转录/翻译反循环 (TTFL) 机制,仍然不完全理解.
- 拉巴胺素 (TOR) 途径的目标,特别是vta和gtr2基因,此前已涉及到 *Neurospora crassa* 中的非TTFL节律性.
研究的目的:
- 为了研究TOR通路在产生和维持*神经状虫*的昼夜节律性中的作用,无论有没有功能性TTFL.
- 开发和验证一种改进的测试方法,用于测量神经菌中的TOR活性.
主要方法:
- 在 *Neurospora crassa* 中开发和验证一种用于TOR活性的新型试验.
- 测量影响TTFL组件 (如*frq7*) 和TOR通路基因 (*vta*, *gtr2*) 的野生类型菌株和突变的TOR活动节律性.
- 在淘汰突变者中分析TOR活动水平 (mesor) 和节奏抑制.
主要成果:
- 在具有功能性TTFL的*神经菌*菌株中,TOR活动表现出强大的昼夜节律性.
- 在TOR节律周期受到TTFL组件突变的影响.
- 在*vta*和*gtr2*淘汰突变体中,TOR节律性受到抑制,它们的平均TOR活性处于野生类型的节律范围内.
- 持续的输出节奏性需要节奏的TOR活动,而不是一个恒定的水平.
结论:
- 拉帕素的目标 (TOR) 途径是昼夜系统中的节律状态变量.
- *Neurospora crassa* 作为一个有价值的模型来研究TOR在昼夜调节中的参与.
- 节律性TOR活动对于维持独立于TTFLs的昼夜输出节律至关重要.
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