Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Next-generation Sequencing03:00

Next-generation Sequencing

97.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.6K
RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

HES1 inhibition overcomes CDK4/6 inhibitor resistance by targeting cancer stemness in lung adenocarcinoma.

Journal of experimental & clinical cancer research : CR·2026
Same author

Microbiota and metabolites modulation of cancer stem cells and chemotherapy sensitivity.

Gut·2026
Same author

Gut microbiome: an emerging player and therapeutic target in cancer.

Cancer biology & medicine·2026
Same author

Gut pathobionts translocate into liver and reshape intrahepatic microbiome to facilitate hepatocellular carcinoma.

Cancer discovery·2026
Same author

The mycobiome, virome and archaeome in gastrointestinal cancers: molecular pathogenesis and therapeutic intervention.

Molecular cancer·2026
Same author

Dietary nitrate drives gastritis by modulating gastric microbiota and metabolites.

Cancer biology & medicine·2026
Same journal

Spinal cord injury induces acute microbiome shock and system-wide transcriptomic reprogramming.

iMeta·2026
Same journal

Chondroitin sulfate restores muscle mass via gut-muscle axis remodeling through sugar-bile acid metabolism reprogramming.

iMeta·2026
Same journal

A large-scale single-cell transcriptomic atlas indicates the immune panorama of influenza A infection.

iMeta·2026
Same journal

Accu16S/AccuITS: Accurate and broadly applicable amplicon sequencing for absolute microbiome quantification.

iMeta·2026
Same journal

Chromatin unwinding state for in situ identification and lineage tracing of circulating tumor cells.

iMeta·2026
Same journal

Two stable gut microbiome guilds predict liver tumor class and treatment responses.

iMeta·2026
查看所有相关文章

相关实验视频

Updated: Jan 7, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.2K

使用滴滴微流体学进行高通量通用单实体测序.

Guoping Wang1, Liuyang Zhao1, Yu Shi1

  • 1Institute of Digestive Diseases and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong Hong Kong SAR China.

iMeta
|December 31, 2025
PubMed
概括
此摘要是机器生成的。

通用单实体测序 (GSE-Seq) 能够对病毒和细菌进行高通量基因组测序. 这种新的框架克服了局限性,揭示了新的微生物物种,并增强了我们对生物多样性的理解.

关键词:
滴滴微流体学 滴滴微流体学长阅读序列编排 长阅读序列编排转基因组学是指转基因组学.微生物暗物质的微生物暗物质.单细胞基因组学是一个单细胞基因组学.单个病毒基因组学

更多相关视频

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

9.0K
Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.4K

相关实验视频

Last Updated: Jan 7, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.2K
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

9.0K
Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.4K

科学领域:

  • 微生物学 微生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 单细胞测序先进的细胞异质性研究.
  • 现有的平台仅限于真核细胞,忽略病毒和细菌.
  • 目前的单实体测序方法存在低吞吐量和基因组碎片化的问题.

研究的目的:

  • 引入通用单实体测序 (GSE-Seq),这是一个新的框架.
  • 克服当前单实体测序技术的局限性.
  • 实现各种生物实体的高通量基因组分析.

主要方法:

  • 集成的工作流程结合了大规模的条形码生成,基于水凝的现场处理和全基因组放大.
  • 在滴滴图书馆准备和长读序列.
  • 适用于人类便和海洋沉积物样本中的病毒群体.

主要成果:

  • 产生了数千个高质量的单实体基因组,其中大多数是新型的.
  • 成功识别了dsDNA,ssDNA,巨型病毒和crAssphages.
  • 揭示了假定新型细菌物种,证明了微生物王国的平台多功能性.

结论:

  • GSE-Seq是一个强大的,高吞吐量框架,用于通用单实体测序.
  • 该平台解决了微生物基因组学的关键挑战.
  • 在各种生物应用中,GSE-Seq对单个实体解卷具有显著的前景.