酶媒介沉重金属使用从Sporosarcina pasteurii提取的尿素
Ali Joukar1, Mohammad Mohammadi1, Gholam Reza Ghezelbash1
1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Iranian journal of biotechnology
|December 31, 2025
概括
这项研究表明,用尿素预化尿素酶增强其沉重金属的能力,如和. 这种环保方法提高了酶的稳定性和效率,以实现可持续的整治.
科学领域:
- 环境科学 环境科学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 重金属 (HM) 污染是一个重大的全球环境挑战.
- 尿素诱导的碳酸盐沉提供了一个可持续的,无微生物的修复策略.
研究的目的:
- 从 *Sporosarcina pasteurii* 评估尿酶,以沉 (Pb2+) 和 (Cd2+).
- 研究是否用尿素预化尿素酶可以增强碳酸盐的产生和保护酶免受重金属的侵害.
主要方法:
- 尿素提取和最佳活动条件 (pH,温度,尿素度) 的表征.
- 在不同度下评估Pb2+和Cd2+的降水效率.
- 评估化前对酶稳定性和碳酸盐沉的影响;使用X射线衍射 (XRD) 进行沉物的表征.
主要成果:
- 在pH8,60°C,用0.25M尿素 (Vmax6.8U mg-1蛋白,Km89mM) 观察到的最佳尿酶活性.
- 预化显著增加了碳酸盐的产量,并减少了重金属诱导的酶失活.
- 在20毫米重金属和250毫米尿素下,Cd2+的最大降水效率为82.2%,Pb2+的最大降水效率为38.1%,形成晶体碳酸盐.
结论:
- 提取的尿酶表现出Cd2+和Pb2+的选择性沉.
- 用尿素进行预化可以提高尿酶稳定性和重金属修复效率.
- 这种方法为重金属污染控制提供了具有成本效益和可持续性的解决方案.
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