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一个多细胞和空间图谱定义了人类膀中的纤维细胞

Briana A Santo, Emily E Fink, Pierre-Emmanuel Desprez

bioRxiv : the preprint server for biology

|December 31, 2025

概括

研究人员创建了成年人膀的详细分子和空间地图,揭示了新的细胞类型及其组织. 本资源通过整合基因表达和空间数据来增强对膀功能和疾病的理解.

科学领域:

泌尿器科泌尿器科泌尿器科泌尿器科
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

膀功能取决于各种组织部分之间的复杂相互作用.
这些区间的高分辨率分子和空间细节尚未得到充分理解.

研究的目的:

创建一个全面的,公开可访问的分子和空间参考图的成年人膀.
为了识别细胞类型,它们的空间组织,和调节机制,如多化 (poly(A)) 异型的使用.

主要方法:

74,694个核的单核RNA测序 (snRNA-seq) 已经完成.
使用Xenium (168,476个细胞) 和Visium的空间转录学.
分析聚A) 异型的使用,以提高细胞类型的分辨率.

主要成果:

在膀中识别了22种不同的细胞类型.
细胞类型的详细空间映射,揭示了分层的纤维细胞.
证明使用多 (A) 异型可提供更高分辨率的 stromal 细胞识别.

结论:

生成的多式参考链接基因异型调节到人体膀的解剖学上下文.
该资源作为未来膀研究,细胞类型注释和疾病分析的框架.

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