莱普通过通过OCN/HIF-1α轴促进糖分解来调节椎间盘化和骨化
Haoxi Li1, Chengqiang Yu1, Zhuhai Li1
1Department of Spine Surgery, the People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Academy of Medical Sciences, Nanning, China.
Journal of cellular and molecular medicine
|December 31, 2025
概括
素 (LEP) 通过促进软骨末端板 (CEP) 细胞化和骨化来加速椎间盘退化 (IDD). 这通过通过OCN/HIF-1α通路刺激糖解发生,这种过程在抑制剂下是可逆的.
科学领域:
- 生物化学 生化学
- 细胞生物学 细胞生物学
- 整形外科 整形外科 整形外科
背景情况:
- 椎间盘退化 (IDD) 涉及软骨末板 (CEP) 细胞化和骨化.
- 在IDD过程中,CEP细胞主要利用糖解进行能量代谢.
- 素 (LEP) 在IDD中的精确机制,特别是它与CEP细胞中的糖解的联系,尚未完全理解.
研究的目的:
- 调查素 (LEP) 在促进椎间盘退化 (IDD) 的潜在机制.
- 探索糖解和OCN/HIF-1α轴在LEP诱导的CEP细胞化和骨化中的作用.
主要方法:
- 建立大鼠IDD模型和LEP治疗CEP细胞的体外培养.
- 使用糖解抑制剂 (2-DG) 和sh-HIF-1α.
- 评估化和骨化标志物 (BMP-2,Sox9,OCN,Runx2,ALP活动,化结节).
- 测量与糖解相关的蛋白质和乳酸水平.
主要成果:
- 剂量依赖的LEP促进了CEP细胞的化和骨化,上调相关指标和骨质生成标志物.
- LEP增加了葡萄糖分解,由葡萄糖分解相关的蛋白质和乳酸的升高证明,这被2-DG扭转了.
- LEP上调了OCN/HIF-1α轴,OCN或HIF-1α的降低抑制了LEP诱导的化和骨化.
结论:
- 素 (LEP) 在IDD中升高,并加速CEP细胞化和骨化.
- 通过OCN/HIF-1α轴,LEP刺激糖解,推动IDD的进展.
- 针对LEP诱导的糖解路径为IDD提供了潜在的治疗策略.
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