过高血糖会加剧由 Porphyromonas gingivalis-lipopolysaccharide 引起的胎盘炎症
María José Bendek1,2, María Luisa Mizgier2, Carolina Rojas2
1Faculty of Medicine, Doctoral Program in Biomedicine, Centre for Biomedical Research and Innovation, Universidad de los Andes, Santiago, Chile.
Journal of periodontology
|December 31, 2025
概括
孕期糖尿病和牙周炎有联系. 暴露于牙周细菌副产品和高葡萄糖会放大胎盘炎症,影响健康和GDM怀孕.
科学领域:
- 生殖生物学 生殖生物学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 流行病学研究将妊娠期糖尿病 (GDM) 与牙周炎联系起来.
- 牙周细菌,如 Porphyromonas gingivalis (Pg) 和它们的脂多糖 (Pg-LPS) 可能会转移到胎盘中.
- 这种转位可能会在高血糖环境中加剧炎症.
研究的目的:
- 评估组合高血糖 (HG) 和Pg-LPS暴露在人类期胎盘扩展体中的促炎作用.
- 调查HG和Pg-LPS对健康和GDM怀孕胎盘炎症通路的影响.
主要方法:
- 使用了来自健康 (n=10) 和GDM (n=5) 怀孕的人类期胎盘扩张物.
- 探索者通过正常血糖,HG,Pg-LPS或HG+Pg-LPS进行刺激.
- 组织学,RT-qPCR,西斑,免疫光学和多重免疫试验被用于分析分子和细胞反应.
主要成果:
- 双重HG+Pg-LPS刺激增加了纤维蛋白缩和调高了Toll-like受体-4 (TLR-4) mRNA表达.
- 随着双刺激,NF-κB酸化,NLRP3炎症酶和促炎细胞因子mRNA (IL-6,IL-1β,TNF-α) 的增加.
- 在健康和GDM实验者中,双重刺激增加了超级细胞因子水平 (IL-6,IL-1β,TNF-α),没有显著的群体间差异.
结论:
- 一个高血糖环境显著放大了对人胎盘扩张物Pg-LPS的炎症反应.
- 高血糖和Pg-LPS协同作用,增强胎盘炎症.
- 这些发现强调了口腔细菌转移在GDM期间胎盘炎症中的作用.
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