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定义的猪微生物群对Gnotobiotic小猪中的急性沙门氏菌病的影响
Igor Splichal1, Katerina Polakova1,2, Marek Sinkora1
1Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czechia.
概括
猪生殖前的小猪具有明确的猪微生物群 (DPM),可以保护它们免受沙门氏菌Typhimurium (S. Typhimurium) 感染. DPM减少了肠球炎的症状,肠道损伤和炎症,促进了动物健康.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 沙门氏菌 (Salmonella enterica) 的一个亚种. 肠道血清 Typhimurium (S. Typhimurium) 在各种物种中引起肠道结肠炎.
- 在研究宿主-微生物群相互作用和病原体殖民化方面,Gnotobiotic动物模型至关重要.
- 了解早期的肠道殖民是预防肠道感染的关键.
研究的目的:
- 调查定义猪微生物群 (DPM) 预殖民对无菌小猪中S. Typhimurium LT2感染的影响.
- 分析S. Typhimurium感染后的主体急性免疫反应和肠道变化.
- 为了确定DPM是否能保护S. Typhimurium诱导的肠球炎.
主要方法:
- 没有细菌的小猪被定义的猪微生物群 (DPM) 殖民.
- 在DPM殖民之后,小猪被S. Typhimurium LT2挑战.
- 评估了宿主免疫反应,包括细胞因子诱导和组织学变化.
- 评估了细菌转位和肠道完整性.
主要成果:
- 单靠DPM殖民并没有诱导肠球炎或改变基线肠道参数.
- S. Typhimurium LT2感染导致了全身转位,肠道损伤和炎症.
- 用DPM进行预殖民显著改善了S. Typhimurium诱导的肠球炎,肠道损伤和炎症反应.
- 可溶性TLR4和TLR2水平发生了变化,这表明它在调节炎症方面发挥了作用.
结论:
- 新生儿肠道的控制初始殖民化与定义的微生物联盟增强了对肠道病原体的耐药性.
- 在DPM的前殖民化提供了对小猪S. Typhimurium感染的保护作用.
- 这一策略通过增加对肠道病原体的抗性来促进年轻动物的繁荣.
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