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Updated: May 7, 2026

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
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Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

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针对小鼠棕色脂肪细胞的向基因操纵和发热评估的协议

Jakub Bunk1, Drishti Soni1, Matthias Calderon2

  • 1Rosalind & Morris Goodman Cancer Institute, McGill University, Montreal, QC H3A 1A3, Canada; Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada.

STAR protocols
|December 31, 2025
PubMed
概括

这项研究提出了一种可复制的工作流程,用于生成Cre-依赖的腺相关病毒 (AAV) 载体,用于小鼠的细胞类型特异性基因传递. 该协议可以评估棕色脂肪细胞的热生成能力.

关键词:
基因表达的基因表达方式遗传学 遗传学 遗传学 是一个代谢 代谢 代谢 代谢分子生物学分子生物学

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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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