对Yersinia pestis中自发串联基因组放大事件的系统分析
Dmitry N Konanov1, Olga N Liubimova2, Alexander V Kovrizhnikov3
1Research Institute for System Biology and Medicine of Rospotrebnadzor, Moscow, Russia.
PloS one
|December 31, 2025
概括
在Yersinia pestis中,由于其重复序列,自发基因组放大事件很常见. 这项研究在超过一半的实验室分离物中发现了并列重复,通常与移动遗传元素和重组有关.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 细菌病原体的产生
背景情况:
- 基因组碎片的协同放大在压力下的细菌中很常见.
- 自发的基因组放大事件是罕见的,并且不太了解.
- 瘟疫病原体Yersinia pestis具有许多短重复序列,使其易于发生基因组重组.
研究的目的:
- 为了研究Yersinia pestis.中的自发基因组放大事件.
- 分析Y. pestis实验室分离物中合重复的流行率和性质.
- 识别具有不稳定的副本数和其潜在的移动性质的区域.
主要方法:
- 11个Y. pestis菌株的全基因组测序.
- 来自序列阅读档案 (SRA) 的一千多个阅读档案的分析.
- 生物信息分析用于识别并列重复,IS元素,rRNA集群和不稳定的基因组区域.
主要成果:
- 超过一半的Y. pestis实验室分离物分析了港口合重复.
- 串联重复主要与多副本插入序列 (IS) 元素相关,这表明RECA依赖的重组.
- 确定了四个具有不稳定副本数的可复制区域,包括一个整合性可调动元素和两个类似于等离子体的新型区域.
结论:
- 耶尔西尼亚瘟疫很容易发生自发的双重基因组放大,比之前描述的要多得多.
- 涉及IS元件的ReCA依赖性重组是这些重新排列的主要驱动因素.
- 识别新的,可能类似于等离子体的不稳定区域为了解Y. pestis基因组的可塑性和进化提供了新的途径.
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