相关实验视频

Updated: Jan 7, 2026

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

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宿主蛋白激酶C:新型的基因激活蛋白激酶 (MAPK) 特定支架调节流感病毒核出口的核核蛋白质复合体

Indrani Das Jana1, Soumik Dey1, Manoj Si1

  • 1Department of Bioscience and Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.

PLoS pathogens
|December 31, 2025
PubMed
概括

蛋白激酶Cαα (PKCα) 作为支架蛋白,桥接ERK2和流感病毒核蛋白 (NP),促进病毒复制. 这种相互作用对VRNP核出口和病毒传播至关重要.

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Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay10:05

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Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells11:20

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells

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相关概念视频

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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MAPK Signaling Cascades

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cAMP-dependent Protein Kinase Pathways

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Nuclear Export01:42

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The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
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Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
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