传染性胃肠炎病毒基因组RNA中的N6-甲基氨酸通过RNA传感器RIG-I的高效识别来抑制其复制
Jianing Chen1, Shengyu Lin1,2, Qianzi Liu1
1State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Journal of virology
|December 31, 2025
概括
通过增强RIG-I免疫传感,N6-甲基氨酸 (m6A) RNA修饰抑制了传染性胃肠炎病毒 (TGEV) 的复制. 这种抗病毒机制突出显示了m6A.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- N6-甲基氨酸 (m6A) 是一种关键的RNA修饰,影响基因表达和RNA代谢.
- 虽然m6A经常有助于病毒复制,但它的抗病毒作用,特别是在Coronaviridae中,越来越多,但在机理上不清楚.
研究的目的:
- 调查传染性胃肠炎病毒 (TGEV) 感染中的m6A修饰的抑制作用和机制.
- 阐明m6A如何影响宿主对TGEV的抗病毒反应.
主要方法:
- 在感染期间分析TGEV RNA和宿主细胞中的m6A水平.
- 对m6A读者蛋白与病毒RNA相互作用的研究.
- 评估m6A对病毒复制和干扰素 (IFN) 基因表达的抑制的影响.
- 通过RIG-I.I.对TGEVRNA传感的评估.
主要成果:
- 在TGEV基因组中存在一种修改,抑制病毒复制.
- TGEV感染会提高宿主m6A水平,特别是在IFN相关基因中,而m6A抑制会减少IFN的表达.
- TGEV RNA 呈现出高m6A比率,促进RIG-I介导的免疫传感和IFN反应激活.
- m6A读者蛋白与TGEVRNA结合,降低了转录稳定性.
结论:
- 高m6TGEVRNA上的修改增强了RIG-I传感,激活了IFN反应并抑制了病毒复制.
- 这项研究揭示了一个新的抗病毒机制,涉及m6A介导的免疫激活对TGEV.
- 这些发现加深了对m6A在病毒感染和宿主防御中的复杂作用的理解.
更多相关视频
相关概念视频
Experimental RNAi
7.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K
RNA Interference
27.7K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.7K
Translational Regulation
503
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
503
Types of RNA
72.4K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
72.4K
siRNA - Small Interfering RNAs
18.3K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.3K
Leaky Scanning
5.6K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K


