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Updated: Jun 18, 2026

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从实时纳米孔数据对单个蛋白质的大小和形状的连续,低延迟估计

Yuanjie Li1, Cuifeng Ying2, Michael Mayer1

  • 1Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg, Switzerland.

Analytical chemistry
|December 31, 2025
PubMed
概括

本研究介绍了一种实时纳米孔数据分析方法,用于快速蛋白质表征和控制. 双滑窗 (TSW) 算法可以在实验期间立即识别和操纵蛋白质.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...