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应用RNA测序对死亡原因的调查:一个试点研究

Naoki Nakao¹, Takahiro Harada², Kazuhiro Murata³

¹Department of Forensic Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

Legal medicine (Tokyo, Japan)

|December 31, 2025

概括

RNA测序 (RNA-seq) 在传统方法失败时,通过识别胰岛素过量和过敏反应等死亡原因的分子签名,为法医调查带来希望.

科学领域:

法医科学法医科学法医科学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

在法医解剖中确定死亡原因是具有挑战性的,因为临床数据有限.
传统的定量逆转录聚合酶连锁反应 (qRT-PCR) 仅限于预选的目标.
RNA测序 (RNA-seq) 提供了全面的转录组概况,但在法医调查中未得到充分利用.

研究的目的:

探索RNA测序 (RNA-seq) 在识别特定死亡原因的分子特征方面的实用性.
展示RNA-seq作为法医诊断中的补充工具的潜力.

主要方法:

应用于RNA测序 (RNA-seq) 两个法医病例:致命的胰岛素过量服用和过敏性休克.
使用相对日志表达正常化,将基因表达特征与对照病例进行比较.

主要成果:

在胰岛素过量服用的情况下,观察到胰岛素受体基质PI3K-PDK1/2通路和相关的代谢级联中转录的上调.
在过敏反应病例中,检测到FcεRI信号和T细胞受体相关分子下游基因的表达增加.

结论:

RNA测序 (RNA-seq) 可以捕获与特定死亡原因相关的独特分子特征.
通过RNA-seq进行转录基因分析显示,它有可能成为传统法医诊断方法的有价值补充.

关键词:

过敏反应 (ANAPHYLAXIS) 是一种过敏反应.死亡原因死亡原因法医解剖的尸体检查.过量的胰岛素过量服用.有关RNA测序的RNA测序文字转录学 (Transcriptomics) 是一个学科.

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