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在共价有机框架中酶固定化,以有效的生物电触媒CO2降解为酸和甘氨酸

Hongling Shi¹, Xichuan Zhang², Feifei Sun²

¹Henan Provincial Engineering Research Center of Insect Bio-reactor and Henan Key Laboratory of Insect Biology, College of Life Science, Nanyang Normal University, 1638 Wolong Road, Nanyang, Henan 473061, People's Republic of China; School of Bioengineering, Dalian University of Technology, 2 Linggong Road, Dalian, Liaoning 116024, People's Republic of China.

Bioorganic chemistry

|December 31, 2025

概括

固定化的酶表现出增强的稳定性,可以将大气中的二氧化碳 (CO2) 转化为有价值的化学物质,如酸和甘氨酸. 这种生物电催化方法提高了酶的可重复使用性和气候解决方案的效率.

科学领域:

生物催化剂和可持续化学
酶固定和稳定酶的固定和稳定.
二氧化碳利用的利用方法

背景情况:

大气中的二氧化碳转化对于减缓气候变化至关重要.
酶式二氧化碳减排提供了一条有希望的通往有价值化学品的途径.
酶的不稳定性限制了生物电催化物的实际应用.

研究的目的:

开发和比较固定酶以提高稳定性和二氧化碳转化.
为了研究不同COF载体对酶不动化的有效性.
建立一个生物电催化系统,用于二氧化碳的酸和甘氨酸生产.

主要方法:

使用PVP辅助的一封装策略合成三个固定酶 (FDH@COF-LZU1,FDH@COF-TpPA,FDH@COF-TpBD).
评估固定酶的稳定性 (热,pH,储存) 和可重复使用性.
建立一个生物电催化系统,用于顺序减排二氧化碳和氨基酸合成,在现场进行NADH再生.

主要成果:

与自由酶相比,固定化的酶表现出优越的热稳定性,pH耐受性,储存稳定性和可重复使用性.
COF-TpPA被证明是多酶固定化的最佳载体,使酸转化为甘氨酸的有效转化.
生物电催化系统在10小时内实现了显著的酸产量 (高达101毫米),并产生了0.51毫米的甘氨酸.

结论:

这项研究提出了一种有效的战略,用于将二氧化碳转化为酸和甘氨酸,使用固定酶.
通过基于COF的固定化来提高酶的稳定性和性能.
这项工作为可持续化学品生产的酶固定化技术提供了宝贵的见解.

关键词:

生物电催化生物电催化二氧化碳是二氧化碳中的一种.形成脱酶的形式固定化固定化固定化多酶级联是多种酶的级联.

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