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在Corynebacterium glutamicum中,低成本乳糖以高产D-塔加的高效单步转化使用L-阿拉比诺斯异构酶

Yun Liu¹, Zhina Qiao¹, Hengwei Zhang¹

¹The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, China; Institute of Future Food Technology, JITRI, Yixing, 214200, China.

International journal of biological macromolecules

|December 31, 2025

概括

这项研究设计了Corynebacterium glutamicum以高效地产生罕见的糖D-塔加. 优化的系统实现了高产量,为工业食品应用铺平了道路.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
食品科学食品科学食品科学

背景情况:

D - 塔加提供健康益处,如降低血糖和抗焦性质.
以前的D-塔加生产方法依赖于大肠杆菌,限制了食品行业的应用.
GRAS (一般公认的安全) 菌株是食品级生产的首选品种.

研究的目的:

使用Corynebacterium glutamicum开发一个高效的D-塔加合成细胞工厂.
增强酶活性和稳定性,以改善D-塔加的产生.
建立食品级D-塔加的高产量工业生产工艺.

主要方法:

从Lactobacillus fermentum中选L-阿拉比诺斯异构酶的检测.
一个最佳的L-阿拉比诺酸异构酶突变体 (D390V/H339P) 的理性设计和工程.
通过RBS优化构建一个双酶合的全细胞系统,通过优化β-galactosidase和L-arabinose异构酶比率.

主要成果:

改造的L-阿拉比诺酸异构酶突变体显示活动增加了40.65%,并改善了温度稳定性.
优化的催化系统从400g/L的乳糖中获得了101.42g/L的D-塔加.
在5L发酵器中达到25.12%的高转化率,代表使用乳糖和GRAS菌株的最高产量.

结论:

科里尼细菌 (Corynebacterium glutamicum) 作为一个有效的底盘,用于D-塔加的生产.
开发的方法为工业规模,食品级的D-塔加生产提供了基础.
这项研究推动了D-塔加在食品工业中的应用.

关键词:

D - 塔加托糖 D - 塔加托糖双酶级联转化系统是双酶级联转化系统.这是GRAS菌株.在L-arabinose异构酶中,蛋白质工程是一种蛋白质工程.在RBS优化方面,RBS的优化

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